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Anti-NT5E/CD73 Rabbit Antibody [C12H2]

Catalog No.: F4081

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:1000 IHC1:100 - 1:400

Application
WB, IHC

Reactivity
Human, Mouse, Rat

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
70 kDa

Positive Control	Human hepatocellular carcinoma; Human lung carcinoma; Human squamous cell carcinoma of the cervix; Human renal cell carcinoma; Normal human spleen; 4T1 synegeneic tumor; CT26 synegeneic tumor; Mouse spleen; Mouse liver, Rat kidney; Rat eye; HT-29; Huh7; HeLa (CoCl2, 0.1 mM, 16 h)
Negative Control

Datasheet & SDS

Biological Description

Specificity
NT5E/CD73 Rabbit mAb detects endogenous levels of total NT5E/CD73 protein.

Clone
C12H2

Synonym(s)
5'-nucleotidase; 5'-deoxynucleotidase 2; Ecto-5'-nucleotidase; IMP-specific 5'-nucleotidase 2; Thymidylate 5'-phosphatase; CD73; 5'-NT; NT5; NTE

Background
NT5E/CD73, is a membrane-bound ecto-5′-nucleotidase that functions as a glycosylphosphatidylinositol (GPI)-anchored homodimer, catalyzing the largely irreversible hydrolysis of extracellular adenosine monophosphate (AMP) into adenosine and inorganic phosphate. Structurally, it comprises N-terminal Zn²⁺-binding and glycosylated domains linked to C-terminal substrate-binding domains via a flexible α-helix, enabling open–closed conformational changes during catalysis. Widely expressed on epithelial cells, endothelial cells, smooth muscle cells, lymphocytes, and regulatory T cells, CD73 plays diverse physiological roles, including regulation of epithelial ion transport, maintenance of vascular integrity, modulation of inflammation, and immune tolerance. Pathologically, its adenosine-generating activity acts as an immune checkpoint by suppressing T cell, NK cell, and dendritic cell functions, thereby facilitating tumor immune evasion, angiogenesis, metastasis, and adaptation to hypoxia. In addition to its enzymatic role, CD73 mediates cell–cell and cell–matrix adhesion, supporting tumor progression through both immunosuppressive and pro-metastatic mechanisms.

Background

NT5E/CD73, is a membrane-bound ecto-5′-nucleotidase that functions as a glycosylphosphatidylinositol (GPI)-anchored homodimer, catalyzing the largely irreversible hydrolysis of extracellular adenosine monophosphate (AMP) into adenosine and inorganic phosphate. Structurally, it comprises N-terminal Zn²⁺-binding and glycosylated domains linked to C-terminal substrate-binding domains via a flexible α-helix, enabling open–closed conformational changes during catalysis. Widely expressed on epithelial cells, endothelial cells, smooth muscle cells, lymphocytes, and regulatory T cells, CD73 plays diverse physiological roles, including regulation of epithelial ion transport, maintenance of vascular integrity, modulation of inflammation, and immune tolerance. Pathologically, its adenosine-generating activity acts as an immune checkpoint by suppressing T cell, NK cell, and dendritic cell functions, thereby facilitating tumor immune evasion, angiogenesis, metastasis, and adaptation to hypoxia. In addition to its enzymatic role, CD73 mediates cell–cell and cell–matrix adhesion, supporting tumor progression through both immunosuppressive and pro-metastatic mechanisms.

References
https://pubmed.ncbi.nlm.nih.gov/29720980/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%