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Anti-Ku80 Rabbit Antibody [B10M12]

Catalog No.: F3874

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000 - 1:10000 IP1:50 IHC1:250 - 1:500 IF1:500 - 1:1000 FCM1:20

Application
WB, IP, IHC, IF, FCM

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
83 kDa 83 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Human tonsil tissue; A549; HeLa; HepG2; MCF7
Negative Control	PC-12; NIH/3T3

Datasheet & SDS

Biological Description

Specificity
Ku80 (B10M12) Rabbit mAb detects endogenous levels of total Ku80 protein.

Clone
B10M12

Synonym(s)
G22P2, XRCC5, X-ray repair cross-complementing protein 5, 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, DNA repair protein XRCC5, Ku80, Ku86, Lupus Ku autoantigen protein p86, Nuclear factor IV, Thyroid-lupus autoantigen, X-ray repair comple

Background
Ku80, encoded by the XRCC5 gene, is a key component of the Ku heterodimer, partnering with Ku70 to mediate the non-homologous end joining (NHEJ) pathway, one of the primary mechanisms for repairing DNA double-strand breaks (DSBs). Ku80 comprises three main domains: an N-terminal von Willebrand A (vWA)-like α/β domain involved in protein-protein interactions, a central β-barrel domain that encircles DNA ends, and a C-terminal α-helical arm that stabilizes interaction with Ku70 and binds the DNA-dependent protein kinase catalytic subunit (DNA-PKcs). The Ku70/Ku80 heterodimer forms a ring-shaped structure that threads onto DNA ends, shielding them from degradation and preventing aberrant processing. Ku80 recognizes a wide variety of DNA ends in a sequence-independent manner and functions as a scaffold to recruit other core NHEJ proteins such as DNA-PKcs, XRCC4, XLF, and DNA ligase IV, thereby facilitating end bridging, processing, and ligation. Ku80 contributes to telomere maintenance, gene silencing near telomeres, and V(D)J recombination essential for immune diversity. Its activity is finely regulated through interactions with repair proteins and post-translational modifications, particularly SUMOylation. Defects or mutations in Ku80 disrupt the efficiency and accuracy of DNA repair, leading to chromosomal instability, impaired immune function, and accelerated aging in various model systems.

Background

Ku80, encoded by the XRCC5 gene, is a key component of the Ku heterodimer, partnering with Ku70 to mediate the non-homologous end joining (NHEJ) pathway, one of the primary mechanisms for repairing DNA double-strand breaks (DSBs). Ku80 comprises three main domains: an N-terminal von Willebrand A (vWA)-like α/β domain involved in protein-protein interactions, a central β-barrel domain that encircles DNA ends, and a C-terminal α-helical arm that stabilizes interaction with Ku70 and binds the DNA-dependent protein kinase catalytic subunit (DNA-PKcs). The Ku70/Ku80 heterodimer forms a ring-shaped structure that threads onto DNA ends, shielding them from degradation and preventing aberrant processing. Ku80 recognizes a wide variety of DNA ends in a sequence-independent manner and functions as a scaffold to recruit other core NHEJ proteins such as DNA-PKcs, XRCC4, XLF, and DNA ligase IV, thereby facilitating end bridging, processing, and ligation. Ku80 contributes to telomere maintenance, gene silencing near telomeres, and V(D)J recombination essential for immune diversity. Its activity is finely regulated through interactions with repair proteins and post-translational modifications, particularly SUMOylation. Defects or mutations in Ku80 disrupt the efficiency and accuracy of DNA repair, leading to chromosomal instability, impaired immune function, and accelerated aging in various model systems.

References
https://pubmed.ncbi.nlm.nih.gov/18164703/ https://pubmed.ncbi.nlm.nih.gov/36762474/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%