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Anti-HMGA2 Rabbit Antibody [A3E2]

Catalog No.: F3770

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 IHC1:1000 IF1:100

Application
WB, IHC, IF

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
12 kDa 18 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Human gastric cancer; Human colon cancer; Human Cervix; HeLa; HepG2 (prepared in 1%SDS Hot lysis method)
Negative Control	Human colon; HepG2 (prepared in RIPA lysis method)

Datasheet & SDS

Biological Description

Specificity
HMGA2 (A3E2) Rabbit mAb detects endogenous levels of total HMGA2 (A3E2) protein.

Clone
A3E2

Synonym(s)
HMGIC, HMGA2, High mobility group protein HMGI-C, High mobility group AT-hook protein 2

Background
High-mobility group AT-hook 2 (HMGA2) is a mammalian non-histone chromosomal protein belonging to the HMGA family, which consists of four members: HMGA1a, HMGA1b, HMGA1c, and HMGA2. The three HMGA1 variants—1a, 1b, and 1c—are generated through alternative splicing of the HMGA1 gene, whereas HMGA2 is encoded by a separate gene, HMGA2. Human HMGA2 is a small DNA-binding protein composed of 109 amino acids and is notable for its asymmetric charge distribution along the protein backbone. This structural feature enables HMGA2 to form homodimers in aqueous solutions. Its C-terminal region comprises 15 amino acids, including seven glutamic acid residues and one aspartic acid residue, along with three serine and two threonine residues that can be phosphorylated by casein kinase 2 (CK2). In mice, HMGA2 is expressed exclusively during early embryonic stages and is absent in adult tissues. In humans, HMGA2 is largely absent from adult tissues except for the lung and kidney, with predominant expression occurring during embryonic and fetal development. Notably, HMGA2 is abundantly expressed in human stem cells, including human embryonic stem (hES) cells and early-stage differentiating embryoid bodies (EBs). High levels are also observed in neural stem cells (NSCs), but expression declines with aging. This reduction is partly attributed to increased levels of microRNA let-7b, which binds to the 3′ UTR of HMGA2 mRNA and suppresses its expression.

Background

High-mobility group AT-hook 2 (HMGA2) is a mammalian non-histone chromosomal protein belonging to the HMGA family, which consists of four members: HMGA1a, HMGA1b, HMGA1c, and HMGA2. The three HMGA1 variants—1a, 1b, and 1c—are generated through alternative splicing of the HMGA1 gene, whereas HMGA2 is encoded by a separate gene, HMGA2. Human HMGA2 is a small DNA-binding protein composed of 109 amino acids and is notable for its asymmetric charge distribution along the protein backbone. This structural feature enables HMGA2 to form homodimers in aqueous solutions. Its C-terminal region comprises 15 amino acids, including seven glutamic acid residues and one aspartic acid residue, along with three serine and two threonine residues that can be phosphorylated by casein kinase 2 (CK2). In mice, HMGA2 is expressed exclusively during early embryonic stages and is absent in adult tissues. In humans, HMGA2 is largely absent from adult tissues except for the lung and kidney, with predominant expression occurring during embryonic and fetal development. Notably, HMGA2 is abundantly expressed in human stem cells, including human embryonic stem (hES) cells and early-stage differentiating embryoid bodies (EBs). High levels are also observed in neural stem cells (NSCs), but expression declines with aging. This reduction is partly attributed to increased levels of microRNA let-7b, which binds to the 3′ UTR of HMGA2 mRNA and suppresses its expression.

References
https://pubmed.ncbi.nlm.nih.gov/32466162/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%