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Anti-Calpain 2 Large Subunit (M-type) Rabbit Antibody [K24F11]

Catalog No.: F4579

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:1000 IP1:50 IF1:200 - 1:400

Application
WB, IP, IF

Reactivity
Human, Mouse, Rat

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
80 kDa

Positive Control	Mouse hippocampus; Mouse colon; PC-3; C6; C2C12
Negative Control	Jurkat

Datasheet & SDS

Biological Description

Specificity
Calpain 2 Large Subunit (M-type) Rabbit mAb detects endogenous levels of total calpain 2 (large subunit) protein.

Clone
K24F11

Synonym(s)
Calpain-2 catalytic subunit, Calcium-activated neutral proteinase 2 (CANP 2), Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain (M-calpain), CAPN2, CANPL2.

Background
Calpains are a family of calcium-dependent intracellular thiol proteases involved in diverse molecular, cellular, and physiological processes. In mammals, the two ubiquitously expressed isoforms are calpain-1 (μ-calpain) and calpain-2 (m-calpain). Both function as heterodimers, consisting of a unique large catalytic subunit of ~80 kDa—encoded by CAPN1 for calpain-1 and CAPN2 for calpain-2—and a shared small regulatory subunit of ~28 kDa encoded by CAPNS1 (also known as CAPN4). Genetic disruption of CAPNS1 eliminates the activity of both calpain-1 and calpain-2, resulting in embryonic lethality. Calpains cleave various cytoskeletal proteins, influencing processes such as cell migration and invasion. In cancer, the calpain system is often dysregulated. Calpain-2 is overexpressed in human colorectal adenocarcinoma. Calpains have pro-survival roles, including the degradation of the tumor suppressor p53 (TP53). They also modulate cell migration by altering, rather than degrading, proteins involved in cell adhesion and cytoskeletal organization. Beyond oncology, calpain-2 dysregulation has been implicated in neurodegeneration. Recent studies show that isoform-specific hyperactivation of calpain-2 occurs at synapses before symptom onset in mouse models of Alzheimer’s disease and is associated with memory impairment in human patients.

Background

Calpains are a family of calcium-dependent intracellular thiol proteases involved in diverse molecular, cellular, and physiological processes. In mammals, the two ubiquitously expressed isoforms are calpain-1 (μ-calpain) and calpain-2 (m-calpain). Both function as heterodimers, consisting of a unique large catalytic subunit of ~80 kDa—encoded by CAPN1 for calpain-1 and CAPN2 for calpain-2—and a shared small regulatory subunit of ~28 kDa encoded by CAPNS1 (also known as CAPN4). Genetic disruption of CAPNS1 eliminates the activity of both calpain-1 and calpain-2, resulting in embryonic lethality. Calpains cleave various cytoskeletal proteins, influencing processes such as cell migration and invasion. In cancer, the calpain system is often dysregulated. Calpain-2 is overexpressed in human colorectal adenocarcinoma. Calpains have pro-survival roles, including the degradation of the tumor suppressor p53 (TP53). They also modulate cell migration by altering, rather than degrading, proteins involved in cell adhesion and cytoskeletal organization. Beyond oncology, calpain-2 dysregulation has been implicated in neurodegeneration. Recent studies show that isoform-specific hyperactivation of calpain-2 occurs at synapses before symptom onset in mouse models of Alzheimer’s disease and is associated with memory impairment in human patients.

References
https://pubmed.ncbi.nlm.nih.gov/22427650/ https://pubmed.ncbi.nlm.nih.gov/30177812/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%