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Anti-Bax Rabbit Antibody [F1C6]

Catalog No.: F3518

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 IP1:200 IF1:100 - 1:200 FCM1:100 - 1:400

Application
WB, IP, IF, FCM

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
20 kDa

Positive Control	MCF-7; A549; HepG2; ACHN; HT-1080
Negative Control	DU 145

Datasheet & SDS

Biological Description

Specificity
Bax (F1C6) Rabbit mAb detects endogenous levels of total Bax (F1C6) protein.

Clone
F1C6

Synonym(s)
Apoptosis regulator BAX, Bcl-2-like protein 4 (Bcl2-L-4), BAX, BCL2L4.

Background
Mitochondria play an essential role in cell survival by producing ATP through oxidative phosphorylation. The pro-apoptotic Bcl-2 family members Bax and Bak are pivotal regulators of programmed cell death. Normally existing as inert monomers, Bax and Bak can undergo activation to form oligomers that create pores in the mitochondrial outer membrane (MOM), triggering apoptosis. Their activity is tightly controlled by other Bcl-2 family proteins: BH3-only proteins directly activate Bax and Bak, while prosurvival Bcl-2 proteins inhibit them through direct binding. Bax, in particular, serves as a critical switch between cell life and death. Under resting conditions, Bax is mainly located in the cytosol in a monomeric, inactive state. Upon cellular stress, it undergoes a conformational change into an oligomeric form capable of permeabilizing the MOM. This structural transformation is a tightly regulated event, as inappropriate Bax activation poses a severe threat to cell viability. Bax balances stability and flexibility: the N- and C-terminal regions have auto-inhibitory features, with the α1–α2 loop covering a key activation site and the C-terminal α9 helix anchored within a regulatory groove. Activation triggers the release of these inhibitory elements. This permeabilization facilitates the release of apoptogenic molecules, including cytochrome c, which then activates caspase-9 and initiates the downstream caspase cascade, irrevocably committing the cell to apoptosis.

Background

Mitochondria play an essential role in cell survival by producing ATP through oxidative phosphorylation. The pro-apoptotic Bcl-2 family members Bax and Bak are pivotal regulators of programmed cell death. Normally existing as inert monomers, Bax and Bak can undergo activation to form oligomers that create pores in the mitochondrial outer membrane (MOM), triggering apoptosis. Their activity is tightly controlled by other Bcl-2 family proteins: BH3-only proteins directly activate Bax and Bak, while prosurvival Bcl-2 proteins inhibit them through direct binding. Bax, in particular, serves as a critical switch between cell life and death. Under resting conditions, Bax is mainly located in the cytosol in a monomeric, inactive state. Upon cellular stress, it undergoes a conformational change into an oligomeric form capable of permeabilizing the MOM. This structural transformation is a tightly regulated event, as inappropriate Bax activation poses a severe threat to cell viability. Bax balances stability and flexibility: the N- and C-terminal regions have auto-inhibitory features, with the α1–α2 loop covering a key activation site and the C-terminal α9 helix anchored within a regulatory groove. Activation triggers the release of these inhibitory elements. This permeabilization facilitates the release of apoptogenic molecules, including cytochrome c, which then activates caspase-9 and initiates the downstream caspase cascade, irrevocably committing the cell to apoptosis.

References
https://pubmed.ncbi.nlm.nih.gov/24162660/ https://pubmed.ncbi.nlm.nih.gov/34389733/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%