Anti-8-Hydroxy-2'-deoxyguanosine Mouse Antibody [K11E8]

Catalog No.: F1714

Application: Reactivity: Species independent

Usage Information

Dilution
IHC1:100 - 1:200

Application
IHC-P

Reactivity
Species independent

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Positive Control	Human colon, Epidermis from hairless mouse
Negative Control

Datasheet & SDS

Biological Description

Specificity
8-Hydroxy-2'-deoxyguanosine Mouse mAb detects both endogenous and exogenous 8-hydroxy-2'-deoxyguanosine (8-OH-dG).

Clone
K11E8

Synonym(s)
8-OH-dG

Background
8-Hydroxy-2'-deoxyguanosine (8-OHdG) is a modified purine 2'-deoxyribonucleoside formed by the hydroxylation of the guanine base at the C8 position in DNA, typically resulting from oxidative damage caused by reactive oxygen species (ROS). Structurally, it consists of a deoxyguanosine molecule with a hydroxyl group at the 8th carbon of the guanine ring. 8-OHdG is widely recognized as a key biomarker of oxidative stress due to its high prevalence among oxidative DNA lesions. It accumulates in both nuclear and mitochondrial DNA and can be measured in various biological samples, including urine, tissues, and leukocyte DNA. Functionally, 8-OHdG serves as an indicator of endogenous DNA damage and has been extensively used to assess the risk and progression of cancer, aging, and degenerative diseases. Its presence reflects cellular exposure to oxidative agents and is instrumental in evaluating the impact of environmental and lifestyle factors on genomic stability.

Background

8-Hydroxy-2'-deoxyguanosine (8-OHdG) is a modified purine 2'-deoxyribonucleoside formed by the hydroxylation of the guanine base at the C8 position in DNA, typically resulting from oxidative damage caused by reactive oxygen species (ROS). Structurally, it consists of a deoxyguanosine molecule with a hydroxyl group at the 8th carbon of the guanine ring. 8-OHdG is widely recognized as a key biomarker of oxidative stress due to its high prevalence among oxidative DNA lesions. It accumulates in both nuclear and mitochondrial DNA and can be measured in various biological samples, including urine, tissues, and leukocyte DNA. Functionally, 8-OHdG serves as an indicator of endogenous DNA damage and has been extensively used to assess the risk and progression of cancer, aging, and degenerative diseases. Its presence reflects cellular exposure to oxidative agents and is instrumental in evaluating the impact of environmental and lifestyle factors on genomic stability.

References
https://pubmed.ncbi.nlm.nih.gov/19412858/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%