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Anti-TIMP3 Rabbit Antibody [M11A20]

Catalog No.: F4065

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 IF1:50 FCM1:500

Application
WB, IF, FCM

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
24 kDa 20 kDa, 25 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-TIMP3 Rabbit Antibody [M11A20] detects endogenous levels of total TIMP3 protein.

Clone
M11A20

Synonym(s)
Metalloproteinase inhibitor 3, Protein MIG-5, Tissue inhibitor of metalloproteinases 3, TIMP-3, TIMP3

Background
Tissue Inhibitor of Metalloproteinase 3 (TIMP3) is a unique member of the TIMP family that binds tightly to the extracellular matrix (ECM) and inhibits a broad spectrum of enzymes, including matrix metalloproteinases (MMPs), a disintegrin and metalloproteinases (ADAMs), and ADAM with thrombospondin motifs (ADAMTSs). TIMP3 consists of an N-terminal domain responsible for its inhibitory activity and a C-terminal domain that influences its binding to ECM components and specific metalloproteinases. TIMP3 regulates ECM turnover, inflammation, angiogenesis, and cell apoptosis by inhibiting proteolytic enzymes that degrade ECM and modulating signaling molecules such as TNF-α through ADAM17 inhibition. Its inhibitory action on ADAM17 and ADAMTS4 also links TIMP3 to controlling processes like tumor progression and osteoarthritis. Regulation of TIMP3 expression is complex, involving transcription factors such as Sp1, AP1, and Smads, and post-transcriptional regulation by numerous microRNAs targeting its long 3’ untranslated region (UTR). Growth factors like TGF-β1 stimulate TIMP3 expression via ERK1/2 and Smad pathways, whereas microRNAs such as miR-205 negatively regulate its levels with implications in cardiovascular and inflammatory diseases. TIMP3 mutations or dysregulation contribute to pathological fibrosis, cancer metastasis, and other ECM-related disorders.

Background

Tissue Inhibitor of Metalloproteinase 3 (TIMP3) is a unique member of the TIMP family that binds tightly to the extracellular matrix (ECM) and inhibits a broad spectrum of enzymes, including matrix metalloproteinases (MMPs), a disintegrin and metalloproteinases (ADAMs), and ADAM with thrombospondin motifs (ADAMTSs). TIMP3 consists of an N-terminal domain responsible for its inhibitory activity and a C-terminal domain that influences its binding to ECM components and specific metalloproteinases. TIMP3 regulates ECM turnover, inflammation, angiogenesis, and cell apoptosis by inhibiting proteolytic enzymes that degrade ECM and modulating signaling molecules such as TNF-α through ADAM17 inhibition. Its inhibitory action on ADAM17 and ADAMTS4 also links TIMP3 to controlling processes like tumor progression and osteoarthritis. Regulation of TIMP3 expression is complex, involving transcription factors such as Sp1, AP1, and Smads, and post-transcriptional regulation by numerous microRNAs targeting its long 3’ untranslated region (UTR). Growth factors like TGF-β1 stimulate TIMP3 expression via ERK1/2 and Smad pathways, whereas microRNAs such as miR-205 negatively regulate its levels with implications in cardiovascular and inflammatory diseases. TIMP3 mutations or dysregulation contribute to pathological fibrosis, cancer metastasis, and other ECM-related disorders.

References
https://pubmed.ncbi.nlm.nih.gov/9352216/ https://pubmed.ncbi.nlm.nih.gov/32612540/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%