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Anti-SLC1A5/ASCT2 Rabbit Antibody [F12D14]

Catalog No.: F4045

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 IP1:30 IHC1:1600 IF1:50 FCM1:40

Application
WB, IP, IHC, IF, FCM

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
56 kDa

Datasheet & SDS

Biological Description

Specificity
Anti-SLC1A5/ASCT2 Rabbit Antibody [F12D14] detects endogenous levels of total SLC1A5/ASCT2 protein.

Clone
F12D14

Synonym(s)
ASCT2, M7V1, RDR, RDRC, SLC1A5, Neutral amino acid transporter B(0), ATB(0), Baboon M7 virus receptor, RD114/simian type D retrovirus receptor, Sodium-dependent neutral amino acid transporter type 2, Solute carrier family 1 member 5

Background
SLC1A5, also known as ASCT2 (Alanine-Serine-Cysteine Transporter 2), is a sodium-dependent neutral amino acid antiporter that plays a crucial role in transporting glutamine, a vital amino acid for rapidly proliferating cells, including those in cancer. ASCT2 belongs to the solute carrier family 1 (SLC1) and features transport and scaffold domains linked by a key ECL2a loop that facilitates conformational changes during the transport cycle. Both inward- and outward-facing conformations have been observed, with the HP2 loop serving as an extracellular gate that controls substrate access. ASCT2 functions through a Na+-dependent antiport mechanism, exchanging extracellular glutamine for intracellular neutral amino acids, which is vital for supporting cellular metabolism, mTORC1 pathway activation, T-cell function, and the proliferation of cancer cells. The transporter is highly expressed in tumors and inflammatory cells, correlating with increased glutamine uptake and enhanced tumor growth. Regulation of ASCT2 involves glycosylation at N163 and N212 for membrane stability and trafficking, along with scaffold protein interactions that modulate transport activity. Targeting SLC1A5 reduces tumor growth and impairing mTORC1 signaling.

Background

SLC1A5, also known as ASCT2 (Alanine-Serine-Cysteine Transporter 2), is a sodium-dependent neutral amino acid antiporter that plays a crucial role in transporting glutamine, a vital amino acid for rapidly proliferating cells, including those in cancer. ASCT2 belongs to the solute carrier family 1 (SLC1) and features transport and scaffold domains linked by a key ECL2a loop that facilitates conformational changes during the transport cycle. Both inward- and outward-facing conformations have been observed, with the HP2 loop serving as an extracellular gate that controls substrate access. ASCT2 functions through a Na+-dependent antiport mechanism, exchanging extracellular glutamine for intracellular neutral amino acids, which is vital for supporting cellular metabolism, mTORC1 pathway activation, T-cell function, and the proliferation of cancer cells. The transporter is highly expressed in tumors and inflammatory cells, correlating with increased glutamine uptake and enhanced tumor growth. Regulation of ASCT2 involves glycosylation at N163 and N212 for membrane stability and trafficking, along with scaffold protein interactions that modulate transport activity. Targeting SLC1A5 reduces tumor growth and impairing mTORC1 signaling.

References
https://pubmed.ncbi.nlm.nih.gov/30234109/ https://pubmed.ncbi.nlm.nih.gov/31580259/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%