Anti-SLC1A5/ASCT2 Rabbit Antibody [F12D14]

Catalog No.: F4045

打印

Biological Description

Specificity Anti-SLC1A5/ASCT2 Rabbit Antibody [F12D14] detects endogenous levels of total SLC1A5/ASCT2 protein.
Background SLC1A5, also known as ASCT2 (Alanine-Serine-Cysteine Transporter 2), is a sodium-dependent neutral amino acid antiporter that plays a crucial role in transporting glutamine, a vital amino acid for rapidly proliferating cells, including those in cancer. ASCT2 belongs to the solute carrier family 1 (SLC1) and features transport and scaffold domains linked by a key ECL2a loop that facilitates conformational changes during the transport cycle. Both inward- and outward-facing conformations have been observed, with the HP2 loop serving as an extracellular gate that controls substrate access. ASCT2 functions through a Na+-dependent antiport mechanism, exchanging extracellular glutamine for intracellular neutral amino acids, which is vital for supporting cellular metabolism, mTORC1 pathway activation, T-cell function, and the proliferation of cancer cells. The transporter is highly expressed in tumors and inflammatory cells, correlating with increased glutamine uptake and enhanced tumor growth. Regulation of ASCT2 involves glycosylation at N163 and N212 for membrane stability and trafficking, along with scaffold protein interactions that modulate transport activity. Targeting SLC1A5 reduces tumor growth and impairing mTORC1 signaling.

Usage Information

Application WB, IP, IHC, IF, FCM Dilution
WB IP IHC IF FCM
1:1000 1:30 1:1600 1:50 1:40
Reactivity Human
Source Rabbit MW 56 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

References

  • https://pubmed.ncbi.nlm.nih.gov/30234109/
  • https://pubmed.ncbi.nlm.nih.gov/31580259/

Application Data