Choose Your Country or Region

Anti-Peripherin Rabbit Antibody [P7B12]

Catalog No.: F3997

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000 IP1:30 IHC1:2000 IF1:100 FCM1:50

Application
WB, IP, IHC, IF , FCM

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
54 kDa 28 kDa,45 kDa,56 kDa,58 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-Peripherin Rabbit Antibody [P7B12] detects endogenous levels of total Peripherin protein.

Clone
P7B12

Synonym(s)
NEF4, PRPH1, PRPH, Peripherin, Neurofilament 4

Background
Peripherin is a class III intermediate filament protein primarily expressed in the peripheral nervous system, particularly in motor and sensory neurons. It plays a critical role in neurite outgrowth, axonal transport, and maintaining axonal stability during myelination. It consists of an α-helical rod domain flanked by non-helical N-terminal and C-terminal domains, which facilitate its assembly into filamentous networks characteristic of intermediate filaments. Multiple isoforms of peripherin arise from alternative splicing, affecting its filament assembly and overall neuronal health. Peripherin stabilizes the cytoskeleton, supports neurite extension, and aids in organelle transport by interacting with proteins like Rab7A and AP-3, which are involved in endosomal and lysosomal trafficking. Its expression is tightly regulated by signaling molecules such as nerve growth factor (NGF), interleukin-6, and leukemia inhibitory factor, via promoter elements and JAK-STAT pathways. Additionally, post-translational modifications, including phosphorylation, influence peripherin’s solubility and filament dynamics. Dysregulation and aggregation of peripherin are implicated in neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and peripheral neuropathies.

Background

Peripherin is a class III intermediate filament protein primarily expressed in the peripheral nervous system, particularly in motor and sensory neurons. It plays a critical role in neurite outgrowth, axonal transport, and maintaining axonal stability during myelination. It consists of an α-helical rod domain flanked by non-helical N-terminal and C-terminal domains, which facilitate its assembly into filamentous networks characteristic of intermediate filaments. Multiple isoforms of peripherin arise from alternative splicing, affecting its filament assembly and overall neuronal health. Peripherin stabilizes the cytoskeleton, supports neurite extension, and aids in organelle transport by interacting with proteins like Rab7A and AP-3, which are involved in endosomal and lysosomal trafficking. Its expression is tightly regulated by signaling molecules such as nerve growth factor (NGF), interleukin-6, and leukemia inhibitory factor, via promoter elements and JAK-STAT pathways. Additionally, post-translational modifications, including phosphorylation, influence peripherin’s solubility and filament dynamics. Dysregulation and aggregation of peripherin are implicated in neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and peripheral neuropathies.

References
https://pubmed.ncbi.nlm.nih.gov/36499746/ https://pubmed.ncbi.nlm.nih.gov/39995075/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%