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Anti-ME3 Rabbit Antibody [H14L5]

Catalog No.: F3349

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000 - 1:10000 IHC1:200

Application
WB, IHC

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
67 kDa 67 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-ME3 Rabbit Antibody [H14L5] detects endogenous levels of total ME3 protein.

Clone
H14L5

Synonym(s)
NADP-ME, Malic enzyme 3, ME3

Background
Mitochondrial Malic Enzyme 3 (ME3) is a mitochondrial NADP+-dependent enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate, producing NADPH that is crucial for maintaining cellular redox balance and supporting anabolic processes like lipid biosynthesis. ME3 is a stable homotetramer composed of four domains (A, B, C, and D), with the active site located between domains B and C, aiding substrate and cofactor binding. Unlike the malic enzyme 2 (ME2) isoform, ME3 operates as a non-allosteric enzyme due to tighter hydrophobic interactions in domain A, ensuring stability in mitochondrial enzymatic activity. ME3 is vital for mitochondrial redox homeostasis by providing NADPH for the detoxification of reactive oxygen species. Additionally, it plays a critical role in glucose-stimulated insulin secretion in pancreatic β-cells, where knockdown of ME3 significantly impairs insulin release. ME3 also supports cancer metabolism by promoting tumor growth and survival through maintenance of redox balance and metabolic flexibility. Regulation of ME3 involves multiple layers, including transcriptional control and post-translational modifications, enabling adaptation to variations in metabolic demand and oxidative stress. ME3 acts as a promising target for therapeutic interventions in metabolic disorders and cancer.

Background

Mitochondrial Malic Enzyme 3 (ME3) is a mitochondrial NADP+-dependent enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate, producing NADPH that is crucial for maintaining cellular redox balance and supporting anabolic processes like lipid biosynthesis. ME3 is a stable homotetramer composed of four domains (A, B, C, and D), with the active site located between domains B and C, aiding substrate and cofactor binding. Unlike the malic enzyme 2 (ME2) isoform, ME3 operates as a non-allosteric enzyme due to tighter hydrophobic interactions in domain A, ensuring stability in mitochondrial enzymatic activity. ME3 is vital for mitochondrial redox homeostasis by providing NADPH for the detoxification of reactive oxygen species. Additionally, it plays a critical role in glucose-stimulated insulin secretion in pancreatic β-cells, where knockdown of ME3 significantly impairs insulin release. ME3 also supports cancer metabolism by promoting tumor growth and survival through maintenance of redox balance and metabolic flexibility. Regulation of ME3 involves multiple layers, including transcriptional control and post-translational modifications, enabling adaptation to variations in metabolic demand and oxidative stress. ME3 acts as a promising target for therapeutic interventions in metabolic disorders and cancer.

References
https://pubmed.ncbi.nlm.nih.gov/25594249/ https://pubmed.ncbi.nlm.nih.gov/39994814/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%