Anti-ME3 Rabbit Antibody [H14L5]

Catalog No.: F3349

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Biological Description

Specificity Anti-ME3 Rabbit Antibody [H14L5] detects endogenous levels of total ME3 protein.
Background Mitochondrial Malic Enzyme 3 (ME3) is a mitochondrial NADP+-dependent enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate, producing NADPH that is crucial for maintaining cellular redox balance and supporting anabolic processes like lipid biosynthesis. ME3 is a stable homotetramer composed of four domains (A, B, C, and D), with the active site located between domains B and C, aiding substrate and cofactor binding. Unlike the malic enzyme 2 (ME2) isoform, ME3 operates as a non-allosteric enzyme due to tighter hydrophobic interactions in domain A, ensuring stability in mitochondrial enzymatic activity. ME3 is vital for mitochondrial redox homeostasis by providing NADPH for the detoxification of reactive oxygen species. Additionally, it plays a critical role in glucose-stimulated insulin secretion in pancreatic β-cells, where knockdown of ME3 significantly impairs insulin release. ME3 also supports cancer metabolism by promoting tumor growth and survival through maintenance of redox balance and metabolic flexibility. Regulation of ME3 involves multiple layers, including transcriptional control and post-translational modifications, enabling adaptation to variations in metabolic demand and oxidative stress. ME3 acts as a promising target for therapeutic interventions in metabolic disorders and cancer.

Usage Information

Application WB, IHC Dilution
WB IHC
1:1000 - 1:10000 1:200
Reactivity Mouse, Rat, Human
Source Rabbit MW 67 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

References

  • https://pubmed.ncbi.nlm.nih.gov/25594249/
  • https://pubmed.ncbi.nlm.nih.gov/39994814/

Application Data