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Anti-KGF/FGF-7 Rabbit Antibody [H7F6]

Catalog No.: F3431

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000 - 1:10000

Application
WB

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
23 kDa 28 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-KGF/FGF-7 Rabbit Antibody [H7F6] detects endogenous levels of total KGF/FGF-7 protein.

Clone
H7F6

Synonym(s)
KGF, FGF7, Fibroblast growth factor 7, FGF-7, Heparin-binding growth factor 7, Keratinocyte growth factor, HBGF-7

Background
Keratinocyte growth factor (KGF), also known as fibroblast growth factor-7 (FGF-7), is a paracrine mitogen secreted by mesenchymal cells in response to pro-inflammatory cytokines and steroid hormones. As a heparin-binding growth factor, KGF/FGF-7 signals specifically through the epithelial splice variant of fibroblast growth factor receptor 2, FGFR2-IIIb. This receptor is predominantly expressed on epithelial cells, making them the exclusive targets of KGF/FGF-7 activity. Evidence from functional assays in cell and organ cultures, as well as in vivo studies, demonstrates that KGF/FGF-7 stimulates proliferation only in epithelial cells from diverse tissues. Its expression is upregulated following epithelial injury, where it plays a pivotal role in tissue repair. In the pancreas, KGF/FGF-7 functions as a strong mitogen for ductal epithelial cells. However, its proliferative effect has also been linked to tumorigenesis. Both KGF/FGF-7 and its receptor FGFR2-IIIb are frequently overexpressed in pancreatic cancer cells, as well as in adjacent acinar and ductal cells. Acting as a stromal-derived mediator, KGF/FGF-7 promotes pancreatic cancer cell proliferation through paracrine signaling. Tumor-associated fibroblasts, known to contribute to cancer progression via paracrine pathways, may secrete KGF/FGF-7, thereby enhancing the growth of neighboring tumor cells. Moreover, KGF/FGF-7 is among the AP-1–regulated genes induced by pro-inflammatory cytokines, further linking it to inflammation-driven tumor biology.

Background

Keratinocyte growth factor (KGF), also known as fibroblast growth factor-7 (FGF-7), is a paracrine mitogen secreted by mesenchymal cells in response to pro-inflammatory cytokines and steroid hormones. As a heparin-binding growth factor, KGF/FGF-7 signals specifically through the epithelial splice variant of fibroblast growth factor receptor 2, FGFR2-IIIb. This receptor is predominantly expressed on epithelial cells, making them the exclusive targets of KGF/FGF-7 activity. Evidence from functional assays in cell and organ cultures, as well as in vivo studies, demonstrates that KGF/FGF-7 stimulates proliferation only in epithelial cells from diverse tissues. Its expression is upregulated following epithelial injury, where it plays a pivotal role in tissue repair. In the pancreas, KGF/FGF-7 functions as a strong mitogen for ductal epithelial cells. However, its proliferative effect has also been linked to tumorigenesis. Both KGF/FGF-7 and its receptor FGFR2-IIIb are frequently overexpressed in pancreatic cancer cells, as well as in adjacent acinar and ductal cells. Acting as a stromal-derived mediator, KGF/FGF-7 promotes pancreatic cancer cell proliferation through paracrine signaling. Tumor-associated fibroblasts, known to contribute to cancer progression via paracrine pathways, may secrete KGF/FGF-7, thereby enhancing the growth of neighboring tumor cells. Moreover, KGF/FGF-7 is among the AP-1–regulated genes induced by pro-inflammatory cytokines, further linking it to inflammation-driven tumor biology.

References
https://pubmed.ncbi.nlm.nih.gov/17200110/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%