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Anti-KDM6A/UTX Rabbit Antibody [J23B17]

Catalog No.: F4032

Application: Reactivity: Mouse, Human

Usage Information

Dilution
WB1:1000 FCM1:50

Application
WB, FCM

Reactivity
Mouse, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
154 kDa 150-160 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-KDM6A/UTX Rabbit Antibody [J23B17] recognizes total endogenous levels of KDM6A/UTX protein.

Clone
J23B17

Synonym(s)
UTX, KDM6A, Lysine-specific demethylase 6A, Histone demethylase UTX, Ubiquitously-transcribed TPR protein on the X chromosome, Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein, [histone H3]-trimethyl-L-lysine(27) demethylase 6A

Background
KDM6A (also known as UTX) is an X chromosome–encoded gene that produces a histone demethylase involved in chromatin remodeling and gene regulation. Its enzymatic activity specifically removes di- and tri-methyl groups from lysine 27 on histone H3 (H3K27me2/3), thereby relieving transcriptional repression, enhancing chromatin accessibility, and facilitating gene expression. In XX individuals, KDM6A consistently escapes X chromosome inactivation across various cell types and developmental stages in both humans and mice, leading to biallelic transcription and consequently higher expression in females. KDM6A plays a fundamental role in tissue- and cell-specific differentiation, as well as in embryonic development, homeotic gene regulation, and cellular reprogramming. Functionally, KDM6A interacts with members of the COMPASS complex, including the MLL3 and MLL4 lysine methyltransferases, to coordinate transcriptional programs. Importantly, mutations in KDM6A and its associated partners are recurrently found in a variety of human cancers, highlighting its significance as both a developmental regulator and a cancer-associated gene.

Background

KDM6A (also known as UTX) is an X chromosome–encoded gene that produces a histone demethylase involved in chromatin remodeling and gene regulation. Its enzymatic activity specifically removes di- and tri-methyl groups from lysine 27 on histone H3 (H3K27me2/3), thereby relieving transcriptional repression, enhancing chromatin accessibility, and facilitating gene expression. In XX individuals, KDM6A consistently escapes X chromosome inactivation across various cell types and developmental stages in both humans and mice, leading to biallelic transcription and consequently higher expression in females. KDM6A plays a fundamental role in tissue- and cell-specific differentiation, as well as in embryonic development, homeotic gene regulation, and cellular reprogramming. Functionally, KDM6A interacts with members of the COMPASS complex, including the MLL3 and MLL4 lysine methyltransferases, to coordinate transcriptional programs. Importantly, mutations in KDM6A and its associated partners are recurrently found in a variety of human cancers, highlighting its significance as both a developmental regulator and a cancer-associated gene.

References
https://pubmed.ncbi.nlm.nih.gov/36268511/ https://pubmed.ncbi.nlm.nih.gov/30753822/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%