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Anti-IGF2BP1/IMP1 Rabbit Antibody [L17E6]

Catalog No.: F4017

Application: Reactivity: Rat, Human, Mouse

Usage Information

Dilution
WB1:1000 IHC1:2000 IF1:50 FCM1:500

Application
WB, IHC, IF , FCM

Reactivity
Rat, Human, Mouse

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
63 kDa 65 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-IGF2BP1/IMP1 Rabbit Antibody [L17E6] detects endogenous levels of total IGF2BP1/IMP1 protein.

Clone
L17E6

Synonym(s)
CRDBP, VICKZ1, ZBP1, IGF2BP1, Insulin-like growth factor 2 mRNA-binding protein 1, IGF2 mRNA-binding protein 1, IMP-1, IMP1, Coding region determinant-binding protein, IGF-II mRNA-binding protein 1, VICKZ family member 1, Zipcode-binding protein 1, CRD-BP, ZBP-1

Background
Insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1), also called IMP1, is an evolutionarily conserved RNA-binding protein involved in post-transcriptional gene regulation through modulation of mRNA stability, localization, and translation. IGF2BP1 contains multiple RNA recognition motifs (RRMs) and K homology (KH) domains, which mediate its selective binding to target mRNAs, often within their 3' untranslated regions, thereby affecting their fate in the cell. IGF2BP1 regulates critical biological processes such as cell proliferation, differentiation, migration, and stem cell maintenance by stabilizing oncogenic and regulatory transcripts, including MYC, β-actin, and others. It assembles into ribonucleoprotein granules that dynamically control mRNA metabolism in response to cellular cues. IGF2BP1 is tightly regulated by phosphorylation at specific residues, impacting its RNA-binding capacity, condensation into ribonucleoprotein particles, and involvement in autophagy regulation. Its deletion enhances autophagy and promotes intestinal regeneration via expansion of facultative intestinal stem cells. IGF2BP1 also plays a key role in maintaining intestinal barrier integrity by stabilizing tight junction components like occludin through direct mRNA binding. Dysregulated IGF2BP1 expression is implicated in cancer progression, metabolic disorders, and inflammatory diseases.

Background

Insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1), also called IMP1, is an evolutionarily conserved RNA-binding protein involved in post-transcriptional gene regulation through modulation of mRNA stability, localization, and translation. IGF2BP1 contains multiple RNA recognition motifs (RRMs) and K homology (KH) domains, which mediate its selective binding to target mRNAs, often within their 3' untranslated regions, thereby affecting their fate in the cell. IGF2BP1 regulates critical biological processes such as cell proliferation, differentiation, migration, and stem cell maintenance by stabilizing oncogenic and regulatory transcripts, including MYC, β-actin, and others. It assembles into ribonucleoprotein granules that dynamically control mRNA metabolism in response to cellular cues. IGF2BP1 is tightly regulated by phosphorylation at specific residues, impacting its RNA-binding capacity, condensation into ribonucleoprotein particles, and involvement in autophagy regulation. Its deletion enhances autophagy and promotes intestinal regeneration via expansion of facultative intestinal stem cells. IGF2BP1 also plays a key role in maintaining intestinal barrier integrity by stabilizing tight junction components like occludin through direct mRNA binding. Dysregulated IGF2BP1 expression is implicated in cancer progression, metabolic disorders, and inflammatory diseases.

References
https://pubmed.ncbi.nlm.nih.gov/38081361/ https://pubmed.ncbi.nlm.nih.gov/39342091/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%