Anti-IGF2BP1/IMP1 Rabbit Antibody [L17E6]

Catalog No.: F4017

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Biological Description

Specificity Anti-IGF2BP1/IMP1 Rabbit Antibody [L17E6] detects endogenous levels of total IGF2BP1/IMP1 protein.
Background Insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1), also called IMP1, is an evolutionarily conserved RNA-binding protein involved in post-transcriptional gene regulation through modulation of mRNA stability, localization, and translation. IGF2BP1 contains multiple RNA recognition motifs (RRMs) and K homology (KH) domains, which mediate its selective binding to target mRNAs, often within their 3' untranslated regions, thereby affecting their fate in the cell. IGF2BP1 regulates critical biological processes such as cell proliferation, differentiation, migration, and stem cell maintenance by stabilizing oncogenic and regulatory transcripts, including MYC, β-actin, and others. It assembles into ribonucleoprotein granules that dynamically control mRNA metabolism in response to cellular cues. IGF2BP1 is tightly regulated by phosphorylation at specific residues, impacting its RNA-binding capacity, condensation into ribonucleoprotein particles, and involvement in autophagy regulation. Its deletion enhances autophagy and promotes intestinal regeneration via expansion of facultative intestinal stem cells. IGF2BP1 also plays a key role in maintaining intestinal barrier integrity by stabilizing tight junction components like occludin through direct mRNA binding. Dysregulated IGF2BP1 expression is implicated in cancer progression, metabolic disorders, and inflammatory diseases.

Usage Information

Application WB, IHC, IF , FCM Dilution
WB IHC IF FCM
1:1000 1:2000 1:50 1:500
Reactivity Rat, Human, Mouse
Source Rabbit MW 63 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

References

  • https://pubmed.ncbi.nlm.nih.gov/38081361/
  • https://pubmed.ncbi.nlm.nih.gov/39342091/

Application Data