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Anti-Histone H3 (di methyl Lys36) Rabbit Antibody [L23A20]

Catalog No.: F3399

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:5000 IHC1:250 IF1:500

Application
WB, IHC, IF, ChIP

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
15 kDa 15 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-Histone H3 (di methyl Lys36) Rabbit Antibody [L23A20] detects endogenous levels of total Histone H3 protein, only when di-methylated on Lys36.

Clone
L23A20

Synonym(s)
H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C

Background
The nucleosome, composed of the four core histones H2A, H2B, H3, and H4, represents the fundamental unit of chromatin. Once regarded as a passive structural framework for DNA packaging, histones are now recognized as highly dynamic proteins that undergo diverse post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Among these, histone methylation plays a central role in defining transcriptionally active and inactive chromatin states and is essential for proper genome regulation during development. Arginine methylation of histones H3 (at residues Arg2, Arg17, and Arg26) and H4 (at Arg3) is generally associated with transcriptional activation and is catalyzed by protein arginine methyltransferases (PRMTs), notably co-activators such as PRMT1 and CARM1 (PRMT4). Lysine methylation predominantly occurs on histone H3 (at Lys4, Lys9, Lys27, Lys36, and Lys79) and histone H4 (at Lys20), and can mediate either gene activation or repression, depending on the site and context. These methyl marks serve as docking signals for chromatin-associated factors that contain specialized methyl-lysine binding motifs, including chromodomains (e.g., HP1, PRC1), PHD fingers (e.g., BPTF, ING2), tudor domains (e.g., 53BP1), and WD40 repeats (e.g., WDR5), thereby coordinating downstream chromatin remodeling and transcriptional regulation.

Background

The nucleosome, composed of the four core histones H2A, H2B, H3, and H4, represents the fundamental unit of chromatin. Once regarded as a passive structural framework for DNA packaging, histones are now recognized as highly dynamic proteins that undergo diverse post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Among these, histone methylation plays a central role in defining transcriptionally active and inactive chromatin states and is essential for proper genome regulation during development. Arginine methylation of histones H3 (at residues Arg2, Arg17, and Arg26) and H4 (at Arg3) is generally associated with transcriptional activation and is catalyzed by protein arginine methyltransferases (PRMTs), notably co-activators such as PRMT1 and CARM1 (PRMT4). Lysine methylation predominantly occurs on histone H3 (at Lys4, Lys9, Lys27, Lys36, and Lys79) and histone H4 (at Lys20), and can mediate either gene activation or repression, depending on the site and context. These methyl marks serve as docking signals for chromatin-associated factors that contain specialized methyl-lysine binding motifs, including chromodomains (e.g., HP1, PRC1), PHD fingers (e.g., BPTF, ING2), tudor domains (e.g., 53BP1), and WD40 repeats (e.g., WDR5), thereby coordinating downstream chromatin remodeling and transcriptional regulation.

References
https://pubmed.ncbi.nlm.nih.gov/15268870/ https://pubmed.ncbi.nlm.nih.gov/15479858/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%