Datasheet

Biological Description

Specificity	Anti-Histone H3 (di methyl Lys36) Rabbit Antibody [L23A20] detects endogenous levels of total Histone H3 protein, only when di-methylated on Lys36.
Background	The nucleosome, composed of the four core histones H2A, H2B, H3, and H4, represents the fundamental unit of chromatin. Once regarded as a passive structural framework for DNA packaging, histones are now recognized as highly dynamic proteins that undergo diverse post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Among these, histone methylation plays a central role in defining transcriptionally active and inactive chromatin states and is essential for proper genome regulation during development. Arginine methylation of histones H3 (at residues Arg2, Arg17, and Arg26) and H4 (at Arg3) is generally associated with transcriptional activation and is catalyzed by protein arginine methyltransferases (PRMTs), notably co-activators such as PRMT1 and CARM1 (PRMT4). Lysine methylation predominantly occurs on histone H3 (at Lys4, Lys9, Lys27, Lys36, and Lys79) and histone H4 (at Lys20), and can mediate either gene activation or repression, depending on the site and context. These methyl marks serve as docking signals for chromatin-associated factors that contain specialized methyl-lysine binding motifs, including chromodomains (e.g., HP1, PRC1), PHD fingers (e.g., BPTF, ING2), tudor domains (e.g., 53BP1), and WD40 repeats (e.g., WDR5), thereby coordinating downstream chromatin remodeling and transcriptional regulation.

Specificity

Anti-Histone H3 (di methyl Lys36) Rabbit Antibody [L23A20] detects endogenous levels of total Histone H3 protein, only when di-methylated on Lys36.

Background

The nucleosome, composed of the four core histones H2A, H2B, H3, and H4, represents the fundamental unit of chromatin. Once regarded as a passive structural framework for DNA packaging, histones are now recognized as highly dynamic proteins that undergo diverse post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Among these, histone methylation plays a central role in defining transcriptionally active and inactive chromatin states and is essential for proper genome regulation during development. Arginine methylation of histones H3 (at residues Arg2, Arg17, and Arg26) and H4 (at Arg3) is generally associated with transcriptional activation and is catalyzed by protein arginine methyltransferases (PRMTs), notably co-activators such as PRMT1 and CARM1 (PRMT4). Lysine methylation predominantly occurs on histone H3 (at Lys4, Lys9, Lys27, Lys36, and Lys79) and histone H4 (at Lys20), and can mediate either gene activation or repression, depending on the site and context. These methyl marks serve as docking signals for chromatin-associated factors that contain specialized methyl-lysine binding motifs, including chromodomains (e.g., HP1, PRC1), PHD fingers (e.g., BPTF, ING2), tudor domains (e.g., 53BP1), and WD40 repeats (e.g., WDR5), thereby coordinating downstream chromatin remodeling and transcriptional regulation.

Usage Information

Application

WB, IHC, IF, ChIP

Dilution

WB	IHC	IF
1:5000	1:250	1:500

Reactivity

Mouse, Rat, Human

Source

Rabbit

MW

15 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/15268870/
https://pubmed.ncbi.nlm.nih.gov/15479858/

Anti-Histone H3 (di methyl Lys36) Rabbit Antibody [L23A20]

Biological Description

Usage Information

References

Application Data