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Anti-HDAC3 Rabbit Antibody [J15M12]

Catalog No.: F4029

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:5000 - 1:10000 IP1:20 - 1:50 IHC1:50 IF1:250-1:500 FCM1:30

Application
WB, IP, IHC, IF, FCM

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
49 kDa 49 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-HDAC3 Rabbit Antibody [J15M12] recognizes total endogenous levels of HDAC3 protein.

Clone
J15M12

Synonym(s)
Histone deacetylase 3, HD3, Protein deacetylase HDAC3, Protein deacylase HDAC3, RPD3-2, SMAP45, HDAC3

Background
Histone deacetylases (HDACs) are enzymes that remove acetyl groups from lysine residues on both histone and non-histone proteins. They are central to transcriptional regulation and are implicated in nearly all chromatin-associated biological processes in eukaryotes. The human class I HDACs, which share homology with the yeast RPD3 protein, include HDAC1, HDAC2, HDAC3, and HDAC8. Among these, HDAC3 functions as a regulator of gene expression by deacetylating histones as well as numerous non-histone substrates. Its activity is tightly controlled through interactions with the corepressors N-CoR and SMRT, forming a functional complex that mediates transcriptional repression. HDAC3 localizes to both the nucleus and cytoplasm and targets a broad range of substrates, including histones H2A, H3, and H4, and non-histone proteins such as RelA, SRY, p53, MEF2, PCAF, and p300/CBP. The HDAC3–N-CoR–SMRT complex is essential for silencing transcription driven by nuclear hormone receptors and other transcription factors, including Rev-Erb, COUP-TF, DAX1, MAD, and Pit-1. Furthermore, HDAC3 enzymatic activity is enhanced by phosphorylation at Ser424 by casein kinase 2 (CK2), while dephosphorylation by protein phosphatase 4 (PP4) reduces its activity.

Background

Histone deacetylases (HDACs) are enzymes that remove acetyl groups from lysine residues on both histone and non-histone proteins. They are central to transcriptional regulation and are implicated in nearly all chromatin-associated biological processes in eukaryotes. The human class I HDACs, which share homology with the yeast RPD3 protein, include HDAC1, HDAC2, HDAC3, and HDAC8. Among these, HDAC3 functions as a regulator of gene expression by deacetylating histones as well as numerous non-histone substrates. Its activity is tightly controlled through interactions with the corepressors N-CoR and SMRT, forming a functional complex that mediates transcriptional repression. HDAC3 localizes to both the nucleus and cytoplasm and targets a broad range of substrates, including histones H2A, H3, and H4, and non-histone proteins such as RelA, SRY, p53, MEF2, PCAF, and p300/CBP. The HDAC3–N-CoR–SMRT complex is essential for silencing transcription driven by nuclear hormone receptors and other transcription factors, including Rev-Erb, COUP-TF, DAX1, MAD, and Pit-1. Furthermore, HDAC3 enzymatic activity is enhanced by phosphorylation at Ser424 by casein kinase 2 (CK2), while dephosphorylation by protein phosphatase 4 (PP4) reduces its activity.

References
https://pubmed.ncbi.nlm.nih.gov/15805470/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%