Datasheet

Biological Description

Specificity	Anti-HDAC3 Rabbit Antibody [J15M12] recognizes total endogenous levels of HDAC3 protein.
Background	Histone deacetylases (HDACs) are enzymes that remove acetyl groups from lysine residues on both histone and non-histone proteins. They are central to transcriptional regulation and are implicated in nearly all chromatin-associated biological processes in eukaryotes. The human class I HDACs, which share homology with the yeast RPD3 protein, include HDAC1, HDAC2, HDAC3, and HDAC8. Among these, HDAC3 functions as a regulator of gene expression by deacetylating histones as well as numerous non-histone substrates. Its activity is tightly controlled through interactions with the corepressors N-CoR and SMRT, forming a functional complex that mediates transcriptional repression. HDAC3 localizes to both the nucleus and cytoplasm and targets a broad range of substrates, including histones H2A, H3, and H4, and non-histone proteins such as RelA, SRY, p53, MEF2, PCAF, and p300/CBP. The HDAC3–N-CoR–SMRT complex is essential for silencing transcription driven by nuclear hormone receptors and other transcription factors, including Rev-Erb, COUP-TF, DAX1, MAD, and Pit-1. Furthermore, HDAC3 enzymatic activity is enhanced by phosphorylation at Ser424 by casein kinase 2 (CK2), while dephosphorylation by protein phosphatase 4 (PP4) reduces its activity.

Specificity

Anti-HDAC3 Rabbit Antibody [J15M12] recognizes total endogenous levels of HDAC3 protein.

Background

Histone deacetylases (HDACs) are enzymes that remove acetyl groups from lysine residues on both histone and non-histone proteins. They are central to transcriptional regulation and are implicated in nearly all chromatin-associated biological processes in eukaryotes. The human class I HDACs, which share homology with the yeast RPD3 protein, include HDAC1, HDAC2, HDAC3, and HDAC8. Among these, HDAC3 functions as a regulator of gene expression by deacetylating histones as well as numerous non-histone substrates. Its activity is tightly controlled through interactions with the corepressors N-CoR and SMRT, forming a functional complex that mediates transcriptional repression. HDAC3 localizes to both the nucleus and cytoplasm and targets a broad range of substrates, including histones H2A, H3, and H4, and non-histone proteins such as RelA, SRY, p53, MEF2, PCAF, and p300/CBP. The HDAC3–N-CoR–SMRT complex is essential for silencing transcription driven by nuclear hormone receptors and other transcription factors, including Rev-Erb, COUP-TF, DAX1, MAD, and Pit-1. Furthermore, HDAC3 enzymatic activity is enhanced by phosphorylation at Ser424 by casein kinase 2 (CK2), while dephosphorylation by protein phosphatase 4 (PP4) reduces its activity.

Usage Information

Application

WB, IP, IHC, IF, FCM

Dilution

WB	IP	IHC	IF	FCM
1:5000 - 1:10000	1:20 - 1:50	1:50	1:250-1:500	1:30

Reactivity

Mouse, Rat, Human

Source

Rabbit

MW

49 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/15805470/

Anti-HDAC3 Rabbit Antibody [J15M12]

Biological Description

Usage Information

References

Application Data