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Anti-HDAC2 Rabbit Antibody [B3K13]

Catalog No.: F4041

Application: Reactivity: Human, Rat, Mouse

Usage Information

Dilution
WB1:2000 IP1:60 IF1:250 - 1:500 FCM1:60 - 1:100

Application
WB, IP, IHC, IF, FCM

Reactivity
Human, Rat, Mouse

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
55 kDa 60 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-HDAC2 Rabbit Antibody [B3K13] recognizes endogenous levels of total HDAC2 protein.

Clone
B3K13

Synonym(s)
Histone deacetylase 2, HD2, Protein deacylase HDAC2, HDAC2

Background
Histone acetyltransferases (HATs) and histone deacetylases (HDACs) maintain the balance of histone acetylation and deacetylation, a dynamic process critical for chromatin organization and gene regulation. Disruption of this equilibrium can lead to abnormal chromatin architecture and impaired chromosome function. HDAC2, a class I HDAC, plays a central role in modulating gene expression, cell signaling, and immune responses. By removing acetyl groups from lysine residues on the N-terminal tails of histones H3 and H4, HDAC2 promotes chromatin compaction and transcriptional silencing. Its dysregulation has been linked to the pathogenesis of renal, cardiovascular, neurological, and pulmonary diseases. HDAC2 often functions as a transcriptional repressor in cooperation with HDAC1, though neither enzyme directly binds DNA. Instead, they are recruited by transcription factors such as E2F proteins in association with pocket proteins (pRb, p107, p130), SP1/SP3, YY1, BRCA1, and p53. Alternatively, HDAC2 acts within large multiprotein assemblies, including the CoREST complex, the NuRD complex, and the SIN3 corepressor complex, which tether to DNA through interactions with sequence-specific transcription factors. These complexes also mediate transcriptional regulation via nuclear receptors. Together with HDAC1, HDAC2 governs the transcription of genes essential for hematopoiesis, epithelial differentiation, cardiac development, and neuronal formation, highlighting its importance as a key regulator of cellular processes.

Background

Histone acetyltransferases (HATs) and histone deacetylases (HDACs) maintain the balance of histone acetylation and deacetylation, a dynamic process critical for chromatin organization and gene regulation. Disruption of this equilibrium can lead to abnormal chromatin architecture and impaired chromosome function. HDAC2, a class I HDAC, plays a central role in modulating gene expression, cell signaling, and immune responses. By removing acetyl groups from lysine residues on the N-terminal tails of histones H3 and H4, HDAC2 promotes chromatin compaction and transcriptional silencing. Its dysregulation has been linked to the pathogenesis of renal, cardiovascular, neurological, and pulmonary diseases. HDAC2 often functions as a transcriptional repressor in cooperation with HDAC1, though neither enzyme directly binds DNA. Instead, they are recruited by transcription factors such as E2F proteins in association with pocket proteins (pRb, p107, p130), SP1/SP3, YY1, BRCA1, and p53. Alternatively, HDAC2 acts within large multiprotein assemblies, including the CoREST complex, the NuRD complex, and the SIN3 corepressor complex, which tether to DNA through interactions with sequence-specific transcription factors. These complexes also mediate transcriptional regulation via nuclear receptors. Together with HDAC1, HDAC2 governs the transcription of genes essential for hematopoiesis, epithelial differentiation, cardiac development, and neuronal formation, highlighting its importance as a key regulator of cellular processes.

References
https://pubmed.ncbi.nlm.nih.gov/33714914/ https://pubmed.ncbi.nlm.nih.gov/34013366/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%