Datasheet

Biological Description

Specificity	Anti-HDAC2 Rabbit Antibody [B3K13] recognizes endogenous levels of total HDAC2 protein.
Background	Histone acetyltransferases (HATs) and histone deacetylases (HDACs) maintain the balance of histone acetylation and deacetylation, a dynamic process critical for chromatin organization and gene regulation. Disruption of this equilibrium can lead to abnormal chromatin architecture and impaired chromosome function. HDAC2, a class I HDAC, plays a central role in modulating gene expression, cell signaling, and immune responses. By removing acetyl groups from lysine residues on the N-terminal tails of histones H3 and H4, HDAC2 promotes chromatin compaction and transcriptional silencing. Its dysregulation has been linked to the pathogenesis of renal, cardiovascular, neurological, and pulmonary diseases. HDAC2 often functions as a transcriptional repressor in cooperation with HDAC1, though neither enzyme directly binds DNA. Instead, they are recruited by transcription factors such as E2F proteins in association with pocket proteins (pRb, p107, p130), SP1/SP3, YY1, BRCA1, and p53. Alternatively, HDAC2 acts within large multiprotein assemblies, including the CoREST complex, the NuRD complex, and the SIN3 corepressor complex, which tether to DNA through interactions with sequence-specific transcription factors. These complexes also mediate transcriptional regulation via nuclear receptors. Together with HDAC1, HDAC2 governs the transcription of genes essential for hematopoiesis, epithelial differentiation, cardiac development, and neuronal formation, highlighting its importance as a key regulator of cellular processes.

Specificity

Anti-HDAC2 Rabbit Antibody [B3K13] recognizes endogenous levels of total HDAC2 protein.

Background

Histone acetyltransferases (HATs) and histone deacetylases (HDACs) maintain the balance of histone acetylation and deacetylation, a dynamic process critical for chromatin organization and gene regulation. Disruption of this equilibrium can lead to abnormal chromatin architecture and impaired chromosome function. HDAC2, a class I HDAC, plays a central role in modulating gene expression, cell signaling, and immune responses. By removing acetyl groups from lysine residues on the N-terminal tails of histones H3 and H4, HDAC2 promotes chromatin compaction and transcriptional silencing. Its dysregulation has been linked to the pathogenesis of renal, cardiovascular, neurological, and pulmonary diseases. HDAC2 often functions as a transcriptional repressor in cooperation with HDAC1, though neither enzyme directly binds DNA. Instead, they are recruited by transcription factors such as E2F proteins in association with pocket proteins (pRb, p107, p130), SP1/SP3, YY1, BRCA1, and p53. Alternatively, HDAC2 acts within large multiprotein assemblies, including the CoREST complex, the NuRD complex, and the SIN3 corepressor complex, which tether to DNA through interactions with sequence-specific transcription factors. These complexes also mediate transcriptional regulation via nuclear receptors. Together with HDAC1, HDAC2 governs the transcription of genes essential for hematopoiesis, epithelial differentiation, cardiac development, and neuronal formation, highlighting its importance as a key regulator of cellular processes.

Usage Information

Application

WB, IP, IHC, IF, FCM

Dilution

WB	IP	IF	FCM
1:2000	1:60	1:250 - 1:500	1:60 - 1:100

Reactivity

Human, Rat, Mouse

Source

Rabbit

MW

55 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/33714914/
https://pubmed.ncbi.nlm.nih.gov/34013366/

Anti-HDAC2 Rabbit Antibody [B3K13]

Biological Description

Usage Information

References

Application Data