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Anti-GCN2 Rabbit Antibody [C18L7]

Catalog No.: F3725

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 - 1:10000 IHC1:100 - 1:250 IF1:250 - 1:500 FCM1:100

Application
WB, IHC, IF, FCM

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
187 kDa 171 kDa, 187 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-GCN2 Rabbit Antibody [C18L7] detects endogenous levels of total GCN2 protein.

Clone
C18L7

Synonym(s)
GCN2, KIAA1338, EIF2AK4, eIF-2-alpha kinase GCN2, Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2-like protein

Background
General control nonderepressible 2 (GCN2), encoded by the gene eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), is a serine/threonine-protein kinase that detects amino acid scarcity by binding to uncharged transfer RNAs (tRNAs). It serves as a key regulator of amino acid metabolism under conditions of nutrient limitation. During amino acid starvation, GCN2 phosphorylates eIF2α, resulting in a global reduction of protein synthesis and the activation of gene expression programs that enable cellular adaptation to stress. More broadly, eukaryotic cells employ the integrated stress response (ISR)—a conserved adaptive pathway—to restore homeostasis under various stress conditions. Through eIF2α phosphorylation, GCN2 suppresses the translation of nonessential proteins, enhances the production of survival-related proteins, and stimulates transcription of stress-responsive genes, including those regulating amino acid transport and antioxidant defenses. Beyond nutrient sensing, GCN2 is implicated in multiple physiological and pathological processes, contributing to systemic homeostasis under diverse stressors. Notably, mutations in GCN2 disrupt vascular and parenchymal remodeling, contributing to the pathogenesis of pulmonary fibrosis in both humans and animal models.

Background

General control nonderepressible 2 (GCN2), encoded by the gene eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), is a serine/threonine-protein kinase that detects amino acid scarcity by binding to uncharged transfer RNAs (tRNAs). It serves as a key regulator of amino acid metabolism under conditions of nutrient limitation. During amino acid starvation, GCN2 phosphorylates eIF2α, resulting in a global reduction of protein synthesis and the activation of gene expression programs that enable cellular adaptation to stress. More broadly, eukaryotic cells employ the integrated stress response (ISR)—a conserved adaptive pathway—to restore homeostasis under various stress conditions. Through eIF2α phosphorylation, GCN2 suppresses the translation of nonessential proteins, enhances the production of survival-related proteins, and stimulates transcription of stress-responsive genes, including those regulating amino acid transport and antioxidant defenses. Beyond nutrient sensing, GCN2 is implicated in multiple physiological and pathological processes, contributing to systemic homeostasis under diverse stressors. Notably, mutations in GCN2 disrupt vascular and parenchymal remodeling, contributing to the pathogenesis of pulmonary fibrosis in both humans and animal models.

References
https://pubmed.ncbi.nlm.nih.gov/36901714/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%