|
Toll Free: (877) 796-6397 -- USA and Canada only -- |
Fax: +1-832-582-8590 Orders: +1-832-582-8158 |
Tech Support: +1-832-582-8158 Ext:3 Please provide your Order Number in the email. |
Biological Description
| Specificity | Anti-GCN2 Rabbit Antibody [C18L7] detects endogenous levels of total GCN2 protein. |
|---|---|
| Background | General control nonderepressible 2 (GCN2), encoded by the gene eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), is a serine/threonine-protein kinase that detects amino acid scarcity by binding to uncharged transfer RNAs (tRNAs). It serves as a key regulator of amino acid metabolism under conditions of nutrient limitation. During amino acid starvation, GCN2 phosphorylates eIF2α, resulting in a global reduction of protein synthesis and the activation of gene expression programs that enable cellular adaptation to stress. More broadly, eukaryotic cells employ the integrated stress response (ISR)—a conserved adaptive pathway—to restore homeostasis under various stress conditions. Through eIF2α phosphorylation, GCN2 suppresses the translation of nonessential proteins, enhances the production of survival-related proteins, and stimulates transcription of stress-responsive genes, including those regulating amino acid transport and antioxidant defenses. Beyond nutrient sensing, GCN2 is implicated in multiple physiological and pathological processes, contributing to systemic homeostasis under diverse stressors. Notably, mutations in GCN2 disrupt vascular and parenchymal remodeling, contributing to the pathogenesis of pulmonary fibrosis in both humans and animal models. |
Usage Information
| Application | WB, IHC, IF, FCM | Dilution |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Reactivity | Human | ||||||||||
| Source | Rabbit | MW | 187 kDa | ||||||||
| Storage Buffer | PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3 | Storage (from the date of receipt) |
-20°C (avoid freeze-thaw cycles), 2 years | ||||||||
References
|