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Anti-GATA1 Rabbit Antibody [C16B1]

Catalog No.: F4021

Application: Reactivity: Human

Usage Information

Dilution
WB1:10000 IP1:70 IHC1:100

Application
WB, IP, IHC, ChIP

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
43 kDa 43 kDa, 45 kDa, 47 kDa, 48 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-GATA1 Rabbit Antibody [C16B1] detects endogenous levels of total GATA1 protein.

Clone
C16B1

Synonym(s)
ERYF1, GF1, GATA1, Erythroid transcription factor, Eryf1, GATA-binding factor 1, NF-E1 DNA-binding protein, GATA-1, GF-1

Background
GATA1 is a master transcription factor crucial for erythroid and megakaryocytic differentiation, regulating gene expression by binding to the DNA consensus sequence WGATAR. It contains four functional domains: two transactivation domains located at the N- and C-termini, and two zinc-finger domains in the central region, the N-finger and C-finger. The C-finger is essential for DNA binding at GATA motifs, while the N-finger stabilizes this binding and mediates interactions with cofactors such as FOG1, aiding in GATA1 homodimerization. GATA1 regulates a wide array of target genes involved in erythroid maturation, including those controlling the cell cycle, apoptosis, and differentiation. It represses transcription of cell cycle promoters, such as Cyclin D2, and induces inhibitors like p18^INK4c and p27^Kip1, promoting erythroid differentiation. GATA1 activity is tightly regulated at multiple levels: alternative translation produces a shorter isoform, GATA1s, with reduced transactivation capacity; post-translational acetylation by P300/CBP enhances its transcriptional activity; and caspase-mediated cleavage regulates its levels during erythroid maturation. GATA1 expression is dynamically regulated during hematopoiesis, peaking in early erythroblasts and being downregulated in later stages to ensure proper cellular homeostasis. Mutations or dysregulation of GATA1 are linked to hematological disorders, such as acute megakaryocytic leukemia in Down syndrome.

Background

GATA1 is a master transcription factor crucial for erythroid and megakaryocytic differentiation, regulating gene expression by binding to the DNA consensus sequence WGATAR. It contains four functional domains: two transactivation domains located at the N- and C-termini, and two zinc-finger domains in the central region, the N-finger and C-finger. The C-finger is essential for DNA binding at GATA motifs, while the N-finger stabilizes this binding and mediates interactions with cofactors such as FOG1, aiding in GATA1 homodimerization. GATA1 regulates a wide array of target genes involved in erythroid maturation, including those controlling the cell cycle, apoptosis, and differentiation. It represses transcription of cell cycle promoters, such as Cyclin D2, and induces inhibitors like p18^INK4c and p27^Kip1, promoting erythroid differentiation. GATA1 activity is tightly regulated at multiple levels: alternative translation produces a shorter isoform, GATA1s, with reduced transactivation capacity; post-translational acetylation by P300/CBP enhances its transcriptional activity; and caspase-mediated cleavage regulates its levels during erythroid maturation. GATA1 expression is dynamically regulated during hematopoiesis, peaking in early erythroblasts and being downregulated in later stages to ensure proper cellular homeostasis. Mutations or dysregulation of GATA1 are linked to hematological disorders, such as acute megakaryocytic leukemia in Down syndrome.

References
https://pubmed.ncbi.nlm.nih.gov/15684376/ https://pubmed.ncbi.nlm.nih.gov/28119852/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%