Datasheet

Biological Description

Specificity	Anti-GATA1 Rabbit Antibody [C16B1] detects endogenous levels of total GATA1 protein.
Background	GATA1 is a master transcription factor crucial for erythroid and megakaryocytic differentiation, regulating gene expression by binding to the DNA consensus sequence WGATAR. It contains four functional domains: two transactivation domains located at the N- and C-termini, and two zinc-finger domains in the central region, the N-finger and C-finger. The C-finger is essential for DNA binding at GATA motifs, while the N-finger stabilizes this binding and mediates interactions with cofactors such as FOG1, aiding in GATA1 homodimerization. GATA1 regulates a wide array of target genes involved in erythroid maturation, including those controlling the cell cycle, apoptosis, and differentiation. It represses transcription of cell cycle promoters, such as Cyclin D2, and induces inhibitors like p18^INK4c and p27^Kip1, promoting erythroid differentiation. GATA1 activity is tightly regulated at multiple levels: alternative translation produces a shorter isoform, GATA1s, with reduced transactivation capacity; post-translational acetylation by P300/CBP enhances its transcriptional activity; and caspase-mediated cleavage regulates its levels during erythroid maturation. GATA1 expression is dynamically regulated during hematopoiesis, peaking in early erythroblasts and being downregulated in later stages to ensure proper cellular homeostasis. Mutations or dysregulation of GATA1 are linked to hematological disorders, such as acute megakaryocytic leukemia in Down syndrome.

Specificity

Anti-GATA1 Rabbit Antibody [C16B1] detects endogenous levels of total GATA1 protein.

Background

GATA1 is a master transcription factor crucial for erythroid and megakaryocytic differentiation, regulating gene expression by binding to the DNA consensus sequence WGATAR. It contains four functional domains: two transactivation domains located at the N- and C-termini, and two zinc-finger domains in the central region, the N-finger and C-finger. The C-finger is essential for DNA binding at GATA motifs, while the N-finger stabilizes this binding and mediates interactions with cofactors such as FOG1, aiding in GATA1 homodimerization. GATA1 regulates a wide array of target genes involved in erythroid maturation, including those controlling the cell cycle, apoptosis, and differentiation. It represses transcription of cell cycle promoters, such as Cyclin D2, and induces inhibitors like p18^INK4c and p27^Kip1, promoting erythroid differentiation. GATA1 activity is tightly regulated at multiple levels: alternative translation produces a shorter isoform, GATA1s, with reduced transactivation capacity; post-translational acetylation by P300/CBP enhances its transcriptional activity; and caspase-mediated cleavage regulates its levels during erythroid maturation. GATA1 expression is dynamically regulated during hematopoiesis, peaking in early erythroblasts and being downregulated in later stages to ensure proper cellular homeostasis. Mutations or dysregulation of GATA1 are linked to hematological disorders, such as acute megakaryocytic leukemia in Down syndrome.

Usage Information

Application

WB, IP, IHC, ChIP

Dilution

WB	IP	IHC
1:10000	1:70	1:100

Reactivity

Human

Source

Rabbit

MW

43 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/15684376/
https://pubmed.ncbi.nlm.nih.gov/28119852/

Anti-GATA1 Rabbit Antibody [C16B1]

Biological Description

Usage Information

References

Application Data