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Anti-CDT1/DUP Rabbit Antibody [H16G9]

Catalog No.: F4006

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 IP1:30 IHC1:100 IF1:100

Application
WB, IP, IHC, IF

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
60 kDa 60 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-CDT1/DUP Rabbit Antibody [H16G9] detects endogenous levels of total CDT1/DUP protein.

Clone
H16G9

Synonym(s)
DNA replication factor Cdt1, Double parked homolog, DUP, CDT1

Background
CDT1 (Chromatin Licensing and DNA Replication Factor 1), also known as DUP in Drosophila, is a pivotal protein involved in the initiation of DNA replication during the cell cycle. Its primary role is in the formation of the pre-replication complex (pre-RC), where it recruits and loads the minichromosome maintenance (MCM) helicase complex onto replication origins. CDT1 interacts directly with the MCM complex, the Origin Recognition Complex (ORC), and CDC6, facilitating the assembly of a functional replication origin. A key feature of CDT1 is its novel PEST domain, which is implicated in regulating chromatin association and preventing DNA re-replication. As a molecular bridge, CDT1 delivers soluble MCM complexes to chromatin-bound ORC/CDC6, ensuring the licensing process is properly executed and DNA replication occurs only once per cell cycle. Additionally, CDT1 contributes to kinetochore–microtubule attachment by interacting with the NDC80 complex, which influences chromosome segregation. To prevent aberrant origin licensing, CDT1 is tightly regulated: it is degraded during S phase via ubiquitin-mediated proteasomal pathways involving E3 ligases CRL4^Cdt2^ and CRL1^Skp2^, while phosphorylation by cyclin-dependent kinases modulates its stability and activity. Moreover, the protein geminin binds CDT1 in S and G2 phases to inhibit its origin licensing function. The expression and activity of CDT1 fluctuate throughout the cell cycle, ensuring proper genome stability and cell division. Dysregulation of CDT1 leads to genomic instability and has been implicated in the development of cancer.

Background

CDT1 (Chromatin Licensing and DNA Replication Factor 1), also known as DUP in Drosophila, is a pivotal protein involved in the initiation of DNA replication during the cell cycle. Its primary role is in the formation of the pre-replication complex (pre-RC), where it recruits and loads the minichromosome maintenance (MCM) helicase complex onto replication origins. CDT1 interacts directly with the MCM complex, the Origin Recognition Complex (ORC), and CDC6, facilitating the assembly of a functional replication origin. A key feature of CDT1 is its novel PEST domain, which is implicated in regulating chromatin association and preventing DNA re-replication. As a molecular bridge, CDT1 delivers soluble MCM complexes to chromatin-bound ORC/CDC6, ensuring the licensing process is properly executed and DNA replication occurs only once per cell cycle. Additionally, CDT1 contributes to kinetochore–microtubule attachment by interacting with the NDC80 complex, which influences chromosome segregation. To prevent aberrant origin licensing, CDT1 is tightly regulated: it is degraded during S phase via ubiquitin-mediated proteasomal pathways involving E3 ligases CRL4^Cdt2^ and CRL1^Skp2^, while phosphorylation by cyclin-dependent kinases modulates its stability and activity. Moreover, the protein geminin binds CDT1 in S and G2 phases to inhibit its origin licensing function. The expression and activity of CDT1 fluctuate throughout the cell cycle, ensuring proper genome stability and cell division. Dysregulation of CDT1 leads to genomic instability and has been implicated in the development of cancer.

References
https://pubmed.ncbi.nlm.nih.gov/30281379/ https://pubmed.ncbi.nlm.nih.gov/28025526/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%