Datasheet

Biological Description

Specificity	Anti-CDT1/DUP Rabbit Antibody [H16G9] detects endogenous levels of total CDT1/DUP protein.
Background	CDT1 (Chromatin Licensing and DNA Replication Factor 1), also known as DUP in Drosophila, is a pivotal protein involved in the initiation of DNA replication during the cell cycle. Its primary role is in the formation of the pre-replication complex (pre-RC), where it recruits and loads the minichromosome maintenance (MCM) helicase complex onto replication origins. CDT1 interacts directly with the MCM complex, the Origin Recognition Complex (ORC), and CDC6, facilitating the assembly of a functional replication origin. A key feature of CDT1 is its novel PEST domain, which is implicated in regulating chromatin association and preventing DNA re-replication. As a molecular bridge, CDT1 delivers soluble MCM complexes to chromatin-bound ORC/CDC6, ensuring the licensing process is properly executed and DNA replication occurs only once per cell cycle. Additionally, CDT1 contributes to kinetochore–microtubule attachment by interacting with the NDC80 complex, which influences chromosome segregation. To prevent aberrant origin licensing, CDT1 is tightly regulated: it is degraded during S phase via ubiquitin-mediated proteasomal pathways involving E3 ligases CRL4^Cdt2^ and CRL1^Skp2^, while phosphorylation by cyclin-dependent kinases modulates its stability and activity. Moreover, the protein geminin binds CDT1 in S and G2 phases to inhibit its origin licensing function. The expression and activity of CDT1 fluctuate throughout the cell cycle, ensuring proper genome stability and cell division. Dysregulation of CDT1 leads to genomic instability and has been implicated in the development of cancer.

Specificity

Anti-CDT1/DUP Rabbit Antibody [H16G9] detects endogenous levels of total CDT1/DUP protein.

Background

CDT1 (Chromatin Licensing and DNA Replication Factor 1), also known as DUP in Drosophila, is a pivotal protein involved in the initiation of DNA replication during the cell cycle. Its primary role is in the formation of the pre-replication complex (pre-RC), where it recruits and loads the minichromosome maintenance (MCM) helicase complex onto replication origins. CDT1 interacts directly with the MCM complex, the Origin Recognition Complex (ORC), and CDC6, facilitating the assembly of a functional replication origin. A key feature of CDT1 is its novel PEST domain, which is implicated in regulating chromatin association and preventing DNA re-replication. As a molecular bridge, CDT1 delivers soluble MCM complexes to chromatin-bound ORC/CDC6, ensuring the licensing process is properly executed and DNA replication occurs only once per cell cycle. Additionally, CDT1 contributes to kinetochore–microtubule attachment by interacting with the NDC80 complex, which influences chromosome segregation. To prevent aberrant origin licensing, CDT1 is tightly regulated: it is degraded during S phase via ubiquitin-mediated proteasomal pathways involving E3 ligases CRL4^Cdt2^ and CRL1^Skp2^, while phosphorylation by cyclin-dependent kinases modulates its stability and activity. Moreover, the protein geminin binds CDT1 in S and G2 phases to inhibit its origin licensing function. The expression and activity of CDT1 fluctuate throughout the cell cycle, ensuring proper genome stability and cell division. Dysregulation of CDT1 leads to genomic instability and has been implicated in the development of cancer.

Usage Information

Application

WB, IP, IHC, IF

Dilution

WB	IP	IHC	IF
1:1000	1:30	1:100	1:100

Reactivity

Human

Source

Rabbit

MW

60 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/30281379/
https://pubmed.ncbi.nlm.nih.gov/28025526/

Anti-CDT1/DUP Rabbit Antibody [H16G9]

Biological Description

Usage Information

References

Application Data