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Anti-CAP1 Rabbit Antibody [C11L24]

Catalog No.: F3956

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 - 1:10000 IHC1:100 - 1:250 FCM1:10 - 1:100

Application
WB, IHC, FCM

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
52 kDa

Datasheet & SDS

Biological Description

Specificity
Anti-CAP1 Rabbit Antibody [C11L24] recognizes total endogenous levels of CAP1 protein.

Clone
C11L24

Synonym(s)
CAP, CAP1, Adenylyl cyclase-associated protein 1, CAP 1

Background
Cyclase-associated proteins (CAPs) are evolutionarily conserved actin-binding proteins found in all eukaryotes. They regulate actin dynamics by engaging in distinct interactions: the N-terminal domain associates with the adenylyl cyclase complex, while the C-terminal region binds directly to monomeric actin. Loss of CAP function disrupts cytoskeletal organization, underscoring its role in actin regulation. Among them, adenylyl cyclase-associated protein 1 (CAP1) is a ubiquitously expressed isoform that modulates cell adhesion through interactions with talin1 and FAK (Focal Adhesion Kinase). Elevated CAP1 expression has been linked to cancer progression and metastasis, including in hepatocellular carcinoma, esophageal squamous cell carcinoma, and epithelial ovarian cancer, where it correlates with proliferation, histological grade, and prognosis. CAP1 also promotes cell migration in mammalian systems, supporting its role in cancer cell motility and invasiveness. In breast cancer, CAP1 influences proliferation via regulation of ERK (Extracellular signal-Regulated Kinase) signaling. Notably, CAP1 depletion alters both FAK and ERK activities, producing opposite adhesion and proliferation phenotypes in metastatic versus non-metastatic breast cancer cells.

Background

Cyclase-associated proteins (CAPs) are evolutionarily conserved actin-binding proteins found in all eukaryotes. They regulate actin dynamics by engaging in distinct interactions: the N-terminal domain associates with the adenylyl cyclase complex, while the C-terminal region binds directly to monomeric actin. Loss of CAP function disrupts cytoskeletal organization, underscoring its role in actin regulation. Among them, adenylyl cyclase-associated protein 1 (CAP1) is a ubiquitously expressed isoform that modulates cell adhesion through interactions with talin1 and FAK (Focal Adhesion Kinase). Elevated CAP1 expression has been linked to cancer progression and metastasis, including in hepatocellular carcinoma, esophageal squamous cell carcinoma, and epithelial ovarian cancer, where it correlates with proliferation, histological grade, and prognosis. CAP1 also promotes cell migration in mammalian systems, supporting its role in cancer cell motility and invasiveness. In breast cancer, CAP1 influences proliferation via regulation of ERK (Extracellular signal-Regulated Kinase) signaling. Notably, CAP1 depletion alters both FAK and ERK activities, producing opposite adhesion and proliferation phenotypes in metastatic versus non-metastatic breast cancer cells.

References
https://pubmed.ncbi.nlm.nih.gov/31151140/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%