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Anti-ATG3 Rabbit Antibody [E19C23]

Catalog No.: F3141

Application: Reactivity: Human

Usage Information

Dilution
WB1:10000 - 1:50000 IHC1:100 - 1:250 IF1:100 - 1:250 FCM1:2000

Application
WB, IHC, IF , FCM

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
36 kDa 40 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-ATG3 Rabbit Antibody [E19C23] detects endogenous levels of total ATG3 protein.

Clone
E19C23

Synonym(s)
APG3, APG3L, ATG3, Ubiquitin-like-conjugating enzyme ATG3, Autophagy-related protein 3, Protein PC3-96, APG3-like, hApg3

Background
ATG3 is a crucial E2-like enzyme in the autophagy pathway, facilitating the conjugation of ATG8/LC3 proteins to phosphatidylethanolamine (PE) on the autophagosomal membrane, a key step for autophagosome formation and elongation. Its structure features a catalytic cysteine essential for forming a thioester intermediate with ATG8/LC3, and flexible N- and C-terminal regions that mediate interactions with partners such as the E1-like enzyme ATG7, the ATG12-ATG5 conjugate, and membranes. ATG3 also participates in noncanonical LC3 lipidation pathways. Its enzymatic activity is tightly regulated by an autoinhibitory switch that alters its conformation upon binding to substrates or partners, ensuring precise control of LC3 lipidation. ATG3 aids in autophagosome biogenesis by promoting membrane aggregation and vesicle formation during autophagosome elongation, linking its membrane-binding properties directly to catalytic activity. ATG3 mediates crosstalk with various cellular pathways, influencing processes such as translation regulation (e.g., eIF5A), apoptosis modulation, and stress responses. Dysregulation of ATG3 impairs autophagy, disrupting cellular homeostasis, survival, and differentiation, which is implicated in tumor progression, pathogen defense, and tissue repair.

Background

ATG3 is a crucial E2-like enzyme in the autophagy pathway, facilitating the conjugation of ATG8/LC3 proteins to phosphatidylethanolamine (PE) on the autophagosomal membrane, a key step for autophagosome formation and elongation. Its structure features a catalytic cysteine essential for forming a thioester intermediate with ATG8/LC3, and flexible N- and C-terminal regions that mediate interactions with partners such as the E1-like enzyme ATG7, the ATG12-ATG5 conjugate, and membranes. ATG3 also participates in noncanonical LC3 lipidation pathways. Its enzymatic activity is tightly regulated by an autoinhibitory switch that alters its conformation upon binding to substrates or partners, ensuring precise control of LC3 lipidation. ATG3 aids in autophagosome biogenesis by promoting membrane aggregation and vesicle formation during autophagosome elongation, linking its membrane-binding properties directly to catalytic activity. ATG3 mediates crosstalk with various cellular pathways, influencing processes such as translation regulation (e.g., eIF5A), apoptosis modulation, and stress responses. Dysregulation of ATG3 impairs autophagy, disrupting cellular homeostasis, survival, and differentiation, which is implicated in tumor progression, pathogen defense, and tissue repair.

References
https://pubmed.ncbi.nlm.nih.gov/34235149/ https://pubmed.ncbi.nlm.nih.gov/37679347/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%