Anti-ATG3 Rabbit Antibody [E19C23]

Catalog No.: F3141

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Biological Description

Specificity Anti-ATG3 Rabbit Antibody [E19C23] detects endogenous levels of total ATG3 protein.
Background ATG3 is a crucial E2-like enzyme in the autophagy pathway, facilitating the conjugation of ATG8/LC3 proteins to phosphatidylethanolamine (PE) on the autophagosomal membrane, a key step for autophagosome formation and elongation. Its structure features a catalytic cysteine essential for forming a thioester intermediate with ATG8/LC3, and flexible N- and C-terminal regions that mediate interactions with partners such as the E1-like enzyme ATG7, the ATG12-ATG5 conjugate, and membranes. ATG3 also participates in noncanonical LC3 lipidation pathways. Its enzymatic activity is tightly regulated by an autoinhibitory switch that alters its conformation upon binding to substrates or partners, ensuring precise control of LC3 lipidation. ATG3 aids in autophagosome biogenesis by promoting membrane aggregation and vesicle formation during autophagosome elongation, linking its membrane-binding properties directly to catalytic activity. ATG3 mediates crosstalk with various cellular pathways, influencing processes such as translation regulation (e.g., eIF5A), apoptosis modulation, and stress responses. Dysregulation of ATG3 impairs autophagy, disrupting cellular homeostasis, survival, and differentiation, which is implicated in tumor progression, pathogen defense, and tissue repair.

Usage Information

Application WB, IHC, IF , FCM Dilution
WB IHC IF FCM
1:10000 - 1:50000 1:100 - 1:250 1:100 - 1:250 1:2000
Reactivity Human
Source Rabbit MW 36 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

References

  • https://pubmed.ncbi.nlm.nih.gov/34235149/
  • https://pubmed.ncbi.nlm.nih.gov/37679347/

Application Data