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Anti-TRIM56 Rabbit Antibody [P3H23]

Catalog No.: F3722

Application: Reactivity: Human

Usage Information

Dilution
WB1:10000 - 1:50000 IHC1:1000 IF1:70 FCM1:20

Application
WB, IHC, IF, FCM

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
81 kDa 81 kDa, 88 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-TRIM56 Rabbit Antibody [P3H23] detetcts endogenous levels of total TRIM56 protein.

Clone
P3H23

Synonym(s)
RNF109, TRIM56, E3 ubiquitin-protein ligase TRIM56, RING finger protein 109, Tripartite motif-containing protein 56

Background
TRIM56, also known as Ring finger protein 109 (RNF109), is a member of the tripartite motif (TRIM) protein family that functions primarily as an E3 ubiquitin ligase, but also exhibits deubiquitinase activity and RNA-binding ability. It is a cytoplasmic protein of 755 amino acids encoded on chromosome 7, containing three conserved domains: an N-terminal RING domain, a B-box domain, and a coiled-coil domain, placing it in the C-V TRIM subfamily. TRIM56 expression is widespread in mammalian tissues and is strongly induced by type I interferons, linking it to antiviral defense. Functionally, TRIM56 regulates innate immune signaling by modulating the TLR and cGAS–STING pathways, thereby promoting interferon and cytokine production. It also exerts direct antiviral effects against diverse RNA and DNA viruses, and has context-dependent roles in cancer, acting as an oncogene in some tumors (e.g., breast cancer, glioma) and a tumor suppressor in others (e.g., ovarian, lung, liver cancers). This dual activity underscores its importance in both antiviral immunity and tumorigenesis.

Background

TRIM56, also known as Ring finger protein 109 (RNF109), is a member of the tripartite motif (TRIM) protein family that functions primarily as an E3 ubiquitin ligase, but also exhibits deubiquitinase activity and RNA-binding ability. It is a cytoplasmic protein of 755 amino acids encoded on chromosome 7, containing three conserved domains: an N-terminal RING domain, a B-box domain, and a coiled-coil domain, placing it in the C-V TRIM subfamily. TRIM56 expression is widespread in mammalian tissues and is strongly induced by type I interferons, linking it to antiviral defense. Functionally, TRIM56 regulates innate immune signaling by modulating the TLR and cGAS–STING pathways, thereby promoting interferon and cytokine production. It also exerts direct antiviral effects against diverse RNA and DNA viruses, and has context-dependent roles in cancer, acting as an oncogene in some tumors (e.g., breast cancer, glioma) and a tumor suppressor in others (e.g., ovarian, lung, liver cancers). This dual activity underscores its importance in both antiviral immunity and tumorigenesis.

References
https://pubmed.ncbi.nlm.nih.gov/36902478/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%