Choose Your Country or Region

Anti-Sumo 1 Rabbit Antibody [G24F22]

Catalog No.: F1114

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:1000 - 1:5000 IP1:20 IHC1:250 IF1:250 - 1:500 FCM1:20 - 1:100 CHIP1:5

Application
WB, IP, IHC, IF, FCM, ChIP

Reactivity
Human, Mouse, Rat

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
12 kDa 12 kDa, 80 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-Sumo 1 Rabbit Antibody [G24F22] detects endogenous levels of total Sumo 1 protein.

Clone
G24F22

Synonym(s)
SMT3C, SMT3H3, UBL1, OK/SW-cl.43, SUMO1, Small ubiquitin-related modifier 1, SUMO-1, GAP-modifying protein 1, SMT3 homolog 3, Sentrin, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Ubiquitin-like protein UBL1, GMP1, Smt3C

Background
SUMO-1 (Small Ubiquitin-like Modifier 1) is a ubiquitin-related protein modifier that covalently attaches to target proteins through a process called sumoylation, thereby altering their stability, localization, and activity without generally targeting them for degradation. Structurally, SUMO-1 shares identity with SUMO-2/3 but lacks the lysine residues necessary for polyubiquitin-like chain formation, which gives it distinct regulatory functions. It is expressed in many tissues and localizes predominantly to the nucleus, where it modifies proteins involved in transcription, DNA repair, chromatin organization, and nuclear transport. A well-characterized function of SUMO-1 is its conjugation to RanGAP1, which promotes RanGAP1 localization to the nuclear pore complex, thereby ensuring efficient nuclear transport. More broadly, SUMO-1 regulates diverse cellular processes, including apoptosis, DNA damage response, and chromatin remodeling, highlighting its essential role as a dynamic regulator of gene expression and genome stability.

Background

SUMO-1 (Small Ubiquitin-like Modifier 1) is a ubiquitin-related protein modifier that covalently attaches to target proteins through a process called sumoylation, thereby altering their stability, localization, and activity without generally targeting them for degradation. Structurally, SUMO-1 shares identity with SUMO-2/3 but lacks the lysine residues necessary for polyubiquitin-like chain formation, which gives it distinct regulatory functions. It is expressed in many tissues and localizes predominantly to the nucleus, where it modifies proteins involved in transcription, DNA repair, chromatin organization, and nuclear transport. A well-characterized function of SUMO-1 is its conjugation to RanGAP1, which promotes RanGAP1 localization to the nuclear pore complex, thereby ensuring efficient nuclear transport. More broadly, SUMO-1 regulates diverse cellular processes, including apoptosis, DNA damage response, and chromatin remodeling, highlighting its essential role as a dynamic regulator of gene expression and genome stability.

References
https://pubmed.ncbi.nlm.nih.gov/23328396/ https://pubmed.ncbi.nlm.nih.gov/9357311/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%