Anti-RCC1 Rabbit Antibody [F6F22]

Catalog No.: F1307

Application: Reactivity: Human, Mouse, Rat, Monkey

Usage Information

Dilution
WB1:1000 IF1:50

Application
WB, IF

Reactivity
Human, Mouse, Rat, Monkey

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
45 kDa

Datasheet & SDS

Biological Description

Specificity
Anti-RCC1 Rabbit Antibody [F6F22] detects endogenous levels of total RCC1 protein.

Clone
F6F22

Synonym(s)
Cell cycle regulatory protein; CHC1; Chromosome condensation protein 1; RanGEF; Regulator of chromosome condensation

Background
RCC1 (Regulator of Chromosome Condensation 1) is a 45-kDa nuclear protein and the sole guanine nucleotide exchange factor (GEF) for the small GTPase Ran, essential for cell cycle progression and nuclear transport. Structurally, RCC1 consists of a short N-terminal domain (NTD) containing a lysine-rich nuclear localization signal (NLS) and a major catalytic domain that adopts a seven-bladed β-propeller fold, enabling direct interaction with histones H2A/H2B and nucleosomal DNA. It is ubiquitously expressed in proliferating cells, where it associates with chromatin throughout the cell cycle. Functionally, RCC1 maintains the Ran-GTP gradient by catalyzing the exchange of GDP for GTP on Ran, thereby regulating nucleocytoplasmic transport, mitotic spindle assembly, nuclear envelope reformation, and chromosome condensation. Proper nuclear and chromatin localization of RCC1 is critical, as it ensures asymmetric Ran-GTP distribution, which underlies its essential role in nuclear organization and cell division.

Background

RCC1 (Regulator of Chromosome Condensation 1) is a 45-kDa nuclear protein and the sole guanine nucleotide exchange factor (GEF) for the small GTPase Ran, essential for cell cycle progression and nuclear transport. Structurally, RCC1 consists of a short N-terminal domain (NTD) containing a lysine-rich nuclear localization signal (NLS) and a major catalytic domain that adopts a seven-bladed β-propeller fold, enabling direct interaction with histones H2A/H2B and nucleosomal DNA. It is ubiquitously expressed in proliferating cells, where it associates with chromatin throughout the cell cycle. Functionally, RCC1 maintains the Ran-GTP gradient by catalyzing the exchange of GDP for GTP on Ran, thereby regulating nucleocytoplasmic transport, mitotic spindle assembly, nuclear envelope reformation, and chromosome condensation. Proper nuclear and chromatin localization of RCC1 is critical, as it ensures asymmetric Ran-GTP distribution, which underlies its essential role in nuclear organization and cell division.

References
https://pubmed.ncbi.nlm.nih.gov/18442486/ https://pubmed.ncbi.nlm.nih.gov/26564799/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%