Choose Your Country or Region

Anti-mTOR Rabbit Antibody [L10A18]

Catalog No.: F2516

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000 - 1:5000 IP1:50 - 1:100 IHC1:400

Application
WB, IP, IHC

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
289 kDa 250 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-mTOR Rabbit Antibody [L10A18] detects endogenous levels of total mTOR protein.

Clone
L10A18

Synonym(s)
FRAP, FRAP1, FRAP2, RAFT1, RAPT1, MTOR, Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, mTOR

Background
mTOR, a serine/threonine kinase belonging to the PI3K-related kinase (PIKK) family, serves as the catalytic core of two distinct complexes: mTOR Complex 1 (mTORC1) and mTOR Complex 2 (mTORC2). mTORC1 is composed of three essential subunits—mTOR, Raptor (regulatory-associated protein of mTOR), and mLST8 (mammalian lethal with Sec13 protein 8, also known as GβL). For cells to grow and proliferate, they must enhance the synthesis of proteins, lipids, and nucleotides while concurrently inhibiting catabolic pathways such as autophagy. mTORC1 orchestrates this balance by integrating environmental cues to regulate anabolic and catabolic processes. One of the primary roles of mTORC1 is to stimulate protein synthesis. It does so by phosphorylating two major downstream effectors: p70 S6 Kinase 1 (S6K1) and eIF4E Binding Protein (4EBP). Specifically, mTORC1 phosphorylates S6K1 at Thr389 within its hydrophobic motif, enabling subsequent activation by PDK1. Beyond protein synthesis, mTORC1 promotes cellular growth by actively repressing autophagy and other catabolic pathways. In contrast, mTORC2 primarily regulates proliferation and survival by phosphorylating members of the AGC kinase family (PKA, PKG, and PKC). Its most critical function is the phosphorylation and activation of Akt, a central effector of insulin/PI3K signaling. Activated Akt drives survival, growth, and proliferation by phosphorylating and inhibiting key substrates, including FoxO1/3a transcription factors, the metabolic regulator GSK3β, and TSC2, an upstream inhibitor of mTORC1. Additionally, mTORC2 activates SGK1, another AGC kinase that influences ion transport and cell survival.

Background

mTOR, a serine/threonine kinase belonging to the PI3K-related kinase (PIKK) family, serves as the catalytic core of two distinct complexes: mTOR Complex 1 (mTORC1) and mTOR Complex 2 (mTORC2). mTORC1 is composed of three essential subunits—mTOR, Raptor (regulatory-associated protein of mTOR), and mLST8 (mammalian lethal with Sec13 protein 8, also known as GβL). For cells to grow and proliferate, they must enhance the synthesis of proteins, lipids, and nucleotides while concurrently inhibiting catabolic pathways such as autophagy. mTORC1 orchestrates this balance by integrating environmental cues to regulate anabolic and catabolic processes. One of the primary roles of mTORC1 is to stimulate protein synthesis. It does so by phosphorylating two major downstream effectors: p70 S6 Kinase 1 (S6K1) and eIF4E Binding Protein (4EBP). Specifically, mTORC1 phosphorylates S6K1 at Thr389 within its hydrophobic motif, enabling subsequent activation by PDK1. Beyond protein synthesis, mTORC1 promotes cellular growth by actively repressing autophagy and other catabolic pathways. In contrast, mTORC2 primarily regulates proliferation and survival by phosphorylating members of the AGC kinase family (PKA, PKG, and PKC). Its most critical function is the phosphorylation and activation of Akt, a central effector of insulin/PI3K signaling. Activated Akt drives survival, growth, and proliferation by phosphorylating and inhibiting key substrates, including FoxO1/3a transcription factors, the metabolic regulator GSK3β, and TSC2, an upstream inhibitor of mTORC1. Additionally, mTORC2 activates SGK1, another AGC kinase that influences ion transport and cell survival.

References
https://pubmed.ncbi.nlm.nih.gov/28283069/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%