Datasheet

Biological Description

Specificity	Anti-mTOR Rabbit Antibody [L10A18] detects endogenous levels of total mTOR protein.
Background	mTOR, a serine/threonine kinase belonging to the PI3K-related kinase (PIKK) family, serves as the catalytic core of two distinct complexes: mTOR Complex 1 (mTORC1) and mTOR Complex 2 (mTORC2). mTORC1 is composed of three essential subunits—mTOR, Raptor (regulatory-associated protein of mTOR), and mLST8 (mammalian lethal with Sec13 protein 8, also known as GβL). For cells to grow and proliferate, they must enhance the synthesis of proteins, lipids, and nucleotides while concurrently inhibiting catabolic pathways such as autophagy. mTORC1 orchestrates this balance by integrating environmental cues to regulate anabolic and catabolic processes. One of the primary roles of mTORC1 is to stimulate protein synthesis. It does so by phosphorylating two major downstream effectors: p70 S6 Kinase 1 (S6K1) and eIF4E Binding Protein (4EBP). Specifically, mTORC1 phosphorylates S6K1 at Thr389 within its hydrophobic motif, enabling subsequent activation by PDK1. Beyond protein synthesis, mTORC1 promotes cellular growth by actively repressing autophagy and other catabolic pathways. In contrast, mTORC2 primarily regulates proliferation and survival by phosphorylating members of the AGC kinase family (PKA, PKG, and PKC). Its most critical function is the phosphorylation and activation of Akt, a central effector of insulin/PI3K signaling. Activated Akt drives survival, growth, and proliferation by phosphorylating and inhibiting key substrates, including FoxO1/3a transcription factors, the metabolic regulator GSK3β, and TSC2, an upstream inhibitor of mTORC1. Additionally, mTORC2 activates SGK1, another AGC kinase that influences ion transport and cell survival.

Specificity

Anti-mTOR Rabbit Antibody [L10A18] detects endogenous levels of total mTOR protein.

Background

mTOR, a serine/threonine kinase belonging to the PI3K-related kinase (PIKK) family, serves as the catalytic core of two distinct complexes: mTOR Complex 1 (mTORC1) and mTOR Complex 2 (mTORC2). mTORC1 is composed of three essential subunits—mTOR, Raptor (regulatory-associated protein of mTOR), and mLST8 (mammalian lethal with Sec13 protein 8, also known as GβL). For cells to grow and proliferate, they must enhance the synthesis of proteins, lipids, and nucleotides while concurrently inhibiting catabolic pathways such as autophagy. mTORC1 orchestrates this balance by integrating environmental cues to regulate anabolic and catabolic processes. One of the primary roles of mTORC1 is to stimulate protein synthesis. It does so by phosphorylating two major downstream effectors: p70 S6 Kinase 1 (S6K1) and eIF4E Binding Protein (4EBP). Specifically, mTORC1 phosphorylates S6K1 at Thr389 within its hydrophobic motif, enabling subsequent activation by PDK1. Beyond protein synthesis, mTORC1 promotes cellular growth by actively repressing autophagy and other catabolic pathways. In contrast, mTORC2 primarily regulates proliferation and survival by phosphorylating members of the AGC kinase family (PKA, PKG, and PKC). Its most critical function is the phosphorylation and activation of Akt, a central effector of insulin/PI3K signaling. Activated Akt drives survival, growth, and proliferation by phosphorylating and inhibiting key substrates, including FoxO1/3a transcription factors, the metabolic regulator GSK3β, and TSC2, an upstream inhibitor of mTORC1. Additionally, mTORC2 activates SGK1, another AGC kinase that influences ion transport and cell survival.

Usage Information

Application

WB, IP, IHC

Dilution

WB	IP	IHC
1:1000 - 1:5000	1:50 - 1:100	1:400

Reactivity

Mouse, Rat, Human

Source

Rabbit

MW

289 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/28283069/

Anti-mTOR Rabbit Antibody [L10A18]

Biological Description

Usage Information

References

Application Data