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Anti-IFI16 Rabbit Antibody [F5E19]

Catalog No.: F1288

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 IP1:200 IHC1:200 - 1:800

Application
WB, IP, IHC

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
85 kDa, 95 kDa, 98 kDa

Datasheet & SDS

Biological Description

Specificity
Anti-IFI16 Rabbit Antibody [F5E19] detects endogenous levels of total IFI16 protein.

Clone
F5E19

Synonym(s)
Gamma-interferon-inducible protein 16, Ifi-16, Interferon-inducible myeloid differentiation transcriptional activator, IFI16, IFNGIP1

Background
IFI16 is a prominent sensor of nuclear pathogenic DNA, initiating innate immune signaling and suppressing viral transcription. It is a nuclear protein belonging to the interferon-inducible HIN-200 family, characterized by an N-terminal PYRIN domain, which is involved in apoptosis, inflammation, and immune responses, and two C-terminal HIN domains, each comprising tightly packed OB-fold subdomains. It is widely expressed in human endothelial, epithelial, and hematopoietic cells, with reduced expression often linked to cancers such as pancreatic, prostate, and breast tumors, suggesting a tumor suppressive role. Structurally, the HIN-A domain of IFI16 binds to the basic C terminus of p53, while the HIN-B domain interacts with its core DNA-binding region, together enhancing p53-DNA complex formation and transcriptional activation. Functionally, IFI16 regulates transcription through protein-protein interactions with p53, pRB, E2F1, and BRCA1, modulates p53 stability, and promotes apoptosis in response to DNA damage, thereby playing a central role in tumor suppression, innate immune signaling, and cellular stress responses.

Background

IFI16 is a prominent sensor of nuclear pathogenic DNA, initiating innate immune signaling and suppressing viral transcription. It is a nuclear protein belonging to the interferon-inducible HIN-200 family, characterized by an N-terminal PYRIN domain, which is involved in apoptosis, inflammation, and immune responses, and two C-terminal HIN domains, each comprising tightly packed OB-fold subdomains. It is widely expressed in human endothelial, epithelial, and hematopoietic cells, with reduced expression often linked to cancers such as pancreatic, prostate, and breast tumors, suggesting a tumor suppressive role. Structurally, the HIN-A domain of IFI16 binds to the basic C terminus of p53, while the HIN-B domain interacts with its core DNA-binding region, together enhancing p53-DNA complex formation and transcriptional activation. Functionally, IFI16 regulates transcription through protein-protein interactions with p53, pRB, E2F1, and BRCA1, modulates p53 stability, and promotes apoptosis in response to DNA damage, thereby playing a central role in tumor suppression, innate immune signaling, and cellular stress responses.

References
https://pubmed.ncbi.nlm.nih.gov/21397192/ https://pubmed.ncbi.nlm.nih.gov/37283074/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%