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Anti-Calcium Pump PMCA4 ATPase Mouse Antibody [E6J21]

Catalog No.: F2336

Application: Reactivity: Human, Cow

Usage Information

Dilution
WB1:1000 IHC1:100 - 1:500 FCMUse 2µg for 10⁶ cells

Application
WB, IHC, IF, FCM

Reactivity
Human, Cow

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Observed MW
140 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-Calcium Pump PMCA4 ATPase Mouse Antibody [E6J21] detects endogenous levels of total PMCA4 ATPase protein. This antibody is specific to the PMCA4 isoform.

Clone
E6J21

Synonym(s)
ATP2B2, MXRA1, ATP2B4, Plasma membrane calcium-transporting ATPase 4, PMCA4, Matrix-remodeling-associated protein 1, Plasma membrane calcium ATPase isoform 4, Plasma membrane calcium pump isoform 4

Background
Plasma membrane calcium ATPases (PMCAs) are essential regulators of calcium-dependent signaling pathways, functioning through interactions with partner proteins. By exporting calcium ions out of the cell, PMCAs maintain low local concentrations of intracellular free calcium, thereby enabling precise control of signaling events within specific cellular subdomains. These pumps are critical for sustaining proper cytoplasmic calcium homeostasis through continuous calcium extrusion into the extracellular space. The PMCA family consists of four isoforms (PMCA1–4), each encoded by a distinct gene. While PMCA1 and PMCA4 are expressed broadly across tissues, PMCA2 and PMCA3 display more restricted expression patterns limited to specialized cells. Beyond calcium clearance, PMCAs achieve functional specificity by engaging their intracellular domains in protein–protein interactions with diverse cytoplasmic partners. Among them, plasma membrane calcium ATPase 4 (PMCA4) has emerged as a key modulator of cancer biology, influencing processes such as cell migration, proliferation, and resistance to apoptosis. Notably, PMCA4 is overexpressed in pancreatic ductal adenocarcinoma (PDAC), where its elevated levels are associated with poor clinical outcomes.

Background

Plasma membrane calcium ATPases (PMCAs) are essential regulators of calcium-dependent signaling pathways, functioning through interactions with partner proteins. By exporting calcium ions out of the cell, PMCAs maintain low local concentrations of intracellular free calcium, thereby enabling precise control of signaling events within specific cellular subdomains. These pumps are critical for sustaining proper cytoplasmic calcium homeostasis through continuous calcium extrusion into the extracellular space. The PMCA family consists of four isoforms (PMCA1–4), each encoded by a distinct gene. While PMCA1 and PMCA4 are expressed broadly across tissues, PMCA2 and PMCA3 display more restricted expression patterns limited to specialized cells. Beyond calcium clearance, PMCAs achieve functional specificity by engaging their intracellular domains in protein–protein interactions with diverse cytoplasmic partners. Among them, plasma membrane calcium ATPase 4 (PMCA4) has emerged as a key modulator of cancer biology, influencing processes such as cell migration, proliferation, and resistance to apoptosis. Notably, PMCA4 is overexpressed in pancreatic ductal adenocarcinoma (PDAC), where its elevated levels are associated with poor clinical outcomes.

References
https://pubmed.ncbi.nlm.nih.gov/21537369/ https://pubmed.ncbi.nlm.nih.gov/31963119/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%