Anti-V5-Tag Rabbit Antibody [K5J22]

Catalog No.: F0016

Application: Reactivity: All Species Expected

Usage Information

Dilution
WB1:1000 IP1:50 IF1:500 - 1:2000 FCM1:400 - 1:1600

Application
WB, IP, IF, FCM

Reactivity
All Species Expected

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Datasheet & SDS

Biological Description

Specificity
Anti-V5-Tag Rabbit Antibody [K5J22] detects recombinant proteins containing the V5 epitope tag.

Clone
K5J22

Background
The V5-tag is a short, 14–amino acid epitope tag originally derived from a prokaryotic source and widely used for recombinant protein detection and purification. Structurally, it contains an equal number of positively and negatively charged residues, yielding a pI of 5.85 and no net charge at physiological pH, with three proline residues that confer a partially ordered and stable core while remaining largely unstructured. Because of its small size, neutrality, and resistance to endogenous protease cleavage, the V5-tag can be fused to proteins without significantly altering their function or localization. It is commonly expressed in mammalian and insect cells without background interference and is detected using antibodies or nanobodies in assays such as ELISA, Western blotting, immunofluorescence, flow cytometry, immunoprecipitation, live-cell imaging, and protein–protein interaction studies.

Background

The V5-tag is a short, 14–amino acid epitope tag originally derived from a prokaryotic source and widely used for recombinant protein detection and purification. Structurally, it contains an equal number of positively and negatively charged residues, yielding a pI of 5.85 and no net charge at physiological pH, with three proline residues that confer a partially ordered and stable core while remaining largely unstructured. Because of its small size, neutrality, and resistance to endogenous protease cleavage, the V5-tag can be fused to proteins without significantly altering their function or localization. It is commonly expressed in mammalian and insect cells without background interference and is detected using antibodies or nanobodies in assays such as ELISA, Western blotting, immunofluorescence, flow cytometry, immunoprecipitation, live-cell imaging, and protein–protein interaction studies.

References
https://pubmed.ncbi.nlm.nih.gov/37517699/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%