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Anti-USP14/TGT C-terminal Rabbit Antibody [P20M18]

Catalog No.: F4047

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000 - 1:10000 IF1:70 FCM1:70

Application
WB, IF, FCM

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
56 kDa 63 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-USP14/TGT C-terminal Rabbit Antibody [P20M18] recognizes endogenous levels of total USP14/TGT C-terminal protein.

Clone
P20M18

Synonym(s)
TGT, USP14, Ubiquitin carboxyl-terminal hydrolase 14, Deubiquitinating enzyme 14, Ubiquitin thioesterase 14, Ubiquitin-specific-processing protease 14

Background
Ubiquitin-specific protease 14 (USP14) is a deubiquitinating enzyme that plays a critical role in regulating proteasome function and cellular protein turnover. By removing ubiquitin residues from polyubiquitin chains, USP14 modulates substrate recognition by the proteasome, which can stabilize target proteins by reducing their degradation. Its interaction with the 26S proteasome, influenced by binding dynamics and post-translational modifications such as phosphorylation, allows USP14 to either inhibit or enhance proteasomal activity. Beyond protein homeostasis, USP14 has been shown to activate NF-κB signaling through IκBα deubiquitination and degradation, contributing to inflammatory responses such as IL-1β–induced chondrocyte dedifferentiation in osteoarthritis. USP14 also functions as a key driver of cancer progression, promoting tumor cell proliferation, migration, and angiogenesis. Dysregulation of USP14 has been reported in multiple cancers, as well as in neurodegenerative diseases and immune disorders, underscoring its importance in health and disease.

Background

Ubiquitin-specific protease 14 (USP14) is a deubiquitinating enzyme that plays a critical role in regulating proteasome function and cellular protein turnover. By removing ubiquitin residues from polyubiquitin chains, USP14 modulates substrate recognition by the proteasome, which can stabilize target proteins by reducing their degradation. Its interaction with the 26S proteasome, influenced by binding dynamics and post-translational modifications such as phosphorylation, allows USP14 to either inhibit or enhance proteasomal activity. Beyond protein homeostasis, USP14 has been shown to activate NF-κB signaling through IκBα deubiquitination and degradation, contributing to inflammatory responses such as IL-1β–induced chondrocyte dedifferentiation in osteoarthritis. USP14 also functions as a key driver of cancer progression, promoting tumor cell proliferation, migration, and angiogenesis. Dysregulation of USP14 has been reported in multiple cancers, as well as in neurodegenerative diseases and immune disorders, underscoring its importance in health and disease.

References
https://pubmed.ncbi.nlm.nih.gov/37917013/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%