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Anti-TORC1 Rabbit Antibody [B21M7]

Catalog No.: F4063

Application: Reactivity: Rat, Human

Usage Information

Dilution
WB1:1000 - 1:5000 FCM1:1000 - 1:10000

Application
WB, FCM

Reactivity
Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
67 kDa 78 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-TORC1 Rabbit Antibody [B21M7] detects endogenous levels of total TORC1 protein.

Clone
B21M7

Synonym(s)
KIAA0616, MECT1, TORC1, WAMTP1, CRTC1, CREB-regulated transcription coactivator 1, Mucoepidermoid carcinoma translocated protein 1, Transducer of regulated cAMP response element-binding protein 1, TORC-1, Transducer of CREB protein 1

Background
Target of Rapamycin Complex 1 (TORC1) is a central, multi-protein kinase complex that integrates various signals, such as nutrient, energy, and growth factor availability, to regulate crucial cellular processes like growth, metabolism, and autophagy. TORC1 consists of the serine/threonine kinase mTOR and several regulatory proteins, including the regulatory-associated protein of mTOR (Raptor), mammalian lethal with SEC13 protein 8 (mLST8), PRAS40, and DEPTOR. These components form a dimeric, rhomboid-shaped complex, with mTOR and Raptor playing key roles in dimerization and substrate recruitment. TORC1 drives cellular anabolic processes by phosphorylating downstream targets such as ribosomal protein S6 kinase 1 (S6K1) and eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1), promoting protein synthesis, lipid biogenesis, and nucleotide synthesis, all critical for cell growth and proliferation. TORC1 also suppresses autophagy by phosphorylating ULK1, thus balancing anabolic and catabolic pathways to maintain cellular homeostasis. The activity of TORC1 is tightly regulated, and amino acid availability is sensed through Rag GTPases and the Ragulator complex, which localize TORC1 to the lysosomal surface for activation. Additionally, growth factors activate TORC1 via the PI3K-Akt-TSC-Rheb pathway, while energy stress inhibits TORC1 through AMP-activated protein kinase (AMPK)-mediated phosphorylation of TSC2. PRAS40 acts as an endogenous inhibitor of TORC1, with its inhibitory function being relieved upon phosphorylation.TORC1 can be inhibited by rapamycin pharmacologically by binding to FKBP12 and the FRB domain of mTOR, thereby disrupting the complex’s activity. Hyperactivation of TORC1 is often implicated in various cancers, metabolic disorders, and age-related diseases.

Background

Target of Rapamycin Complex 1 (TORC1) is a central, multi-protein kinase complex that integrates various signals, such as nutrient, energy, and growth factor availability, to regulate crucial cellular processes like growth, metabolism, and autophagy. TORC1 consists of the serine/threonine kinase mTOR and several regulatory proteins, including the regulatory-associated protein of mTOR (Raptor), mammalian lethal with SEC13 protein 8 (mLST8), PRAS40, and DEPTOR. These components form a dimeric, rhomboid-shaped complex, with mTOR and Raptor playing key roles in dimerization and substrate recruitment. TORC1 drives cellular anabolic processes by phosphorylating downstream targets such as ribosomal protein S6 kinase 1 (S6K1) and eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1), promoting protein synthesis, lipid biogenesis, and nucleotide synthesis, all critical for cell growth and proliferation. TORC1 also suppresses autophagy by phosphorylating ULK1, thus balancing anabolic and catabolic pathways to maintain cellular homeostasis. The activity of TORC1 is tightly regulated, and amino acid availability is sensed through Rag GTPases and the Ragulator complex, which localize TORC1 to the lysosomal surface for activation. Additionally, growth factors activate TORC1 via the PI3K-Akt-TSC-Rheb pathway, while energy stress inhibits TORC1 through AMP-activated protein kinase (AMPK)-mediated phosphorylation of TSC2. PRAS40 acts as an endogenous inhibitor of TORC1, with its inhibitory function being relieved upon phosphorylation.TORC1 can be inhibited by rapamycin pharmacologically by binding to FKBP12 and the FRB domain of mTOR, thereby disrupting the complex’s activity. Hyperactivation of TORC1 is often implicated in various cancers, metabolic disorders, and age-related diseases.

References
https://pubmed.ncbi.nlm.nih.gov/35561748/ https://pubmed.ncbi.nlm.nih.gov/20491627/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%