Anti-Thymidylate Synthase Rabbit Antibody [E9D11]

Catalog No.: F4033

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000 - 1:10000 IP1:10 - 1:100 IHC1:50 - 1:100 IF1:100 - 1:250 FCM1:10 - 1:100

Application
WB, IP, IHC, IF, FCM

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
36 kDa

Datasheet & SDS

Biological Description

Specificity
Anti-Thymidylate Synthase Rabbit Antibody [E9D11] recognizes total endogenous levels of Thymidylate Synthase protein.

Clone
E9D11

Synonym(s)
TS, OK/SW-cl.29, TYMS, Thymidylate synthase, TSase

Background
Thymidylate synthase (TS) catalyzes the reductive methylation of deoxyuridine monophosphate (dUMP) to thymidylate (TMP), using 5,10-methylenetetrahydrofolate as the methyl donor. This reaction represents the sole de novo pathway for intracellular TMP production, a nucleotide that is indispensable for DNA synthesis and repair. Inhibition of TS results in thymidylate depletion, triggering thymineless death—a process marked by extensive DNA damage, chromosomal abnormalities, and apoptosis. The anticancer drug 5-fluorouracil (5-FU) functions as a TS inhibitor and has demonstrated efficacy against various solid tumors, including those of the colon, breast, head, and neck. Clinical studies have shown that tumors with low TS expression are more responsive to 5-FU therapy compared to those with high TS levels. Beyond its role as a therapeutic target, TS is essential for cell proliferation and survival, and its expression has been associated with multiple cancer types.

Background

Thymidylate synthase (TS) catalyzes the reductive methylation of deoxyuridine monophosphate (dUMP) to thymidylate (TMP), using 5,10-methylenetetrahydrofolate as the methyl donor. This reaction represents the sole de novo pathway for intracellular TMP production, a nucleotide that is indispensable for DNA synthesis and repair. Inhibition of TS results in thymidylate depletion, triggering thymineless death—a process marked by extensive DNA damage, chromosomal abnormalities, and apoptosis. The anticancer drug 5-fluorouracil (5-FU) functions as a TS inhibitor and has demonstrated efficacy against various solid tumors, including those of the colon, breast, head, and neck. Clinical studies have shown that tumors with low TS expression are more responsive to 5-FU therapy compared to those with high TS levels. Beyond its role as a therapeutic target, TS is essential for cell proliferation and survival, and its expression has been associated with multiple cancer types.

References
https://pubmed.ncbi.nlm.nih.gov/28634233/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%