Anti-STF-1 Rabbit Antibody [E20A22]

Catalog No.: F1390

Application: Reactivity: Human, Mouse

Usage Information

Dilution
WB1:1000 IP1:100 IF1:100 CHIP1:50

Application
WB, IP, IF, ChIP

Reactivity
Human, Mouse

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
50 kDa

Datasheet & SDS

Biological Description

Specificity
Anti-STF-1 Rabbit Antibody [E20A22] detects endogenous levels of total STF-1 protein.

Clone
E20A22

Synonym(s)
Nuclear Hormone Receptors; Nuclear Receptors; SF-1; SF1; STF-1; STF1

Background
STF-1 (Steroidogenic factor 1, also called Ad4BP and encoded by the NR5A1 gene) is an orphan nuclear receptor and transcription factor essential for endocrine development and steroid hormone biosynthesis. Structurally, it possesses a zinc finger DNA-binding domain, a ligand-binding domain, activation domains (AF-1/AF-2), a hinge region, and a fushi tarazu factor 1 (FTZ-F1) box that aids DNA recognition, and its activity is further regulated by phosphorylation, SUMOylation, and acetylation. SF-1 is expressed in the adrenal cortex, gonads, ventromedial hypothalamus, and pituitary gonadotropes from early embryonic stages, where it directs the transcription of genes encoding steroidogenic enzymes such as cytochrome P450 hydroxylases. Functionally, SF-1 is indispensable for adrenal and gonadal development, sex differentiation, pituitary gonadotropin production, and hypothalamic function, with mutations in NR5A1 linked to adrenal insufficiency, gonadal failure, and reproductive disorders.

Background

STF-1 (Steroidogenic factor 1, also called Ad4BP and encoded by the NR5A1 gene) is an orphan nuclear receptor and transcription factor essential for endocrine development and steroid hormone biosynthesis. Structurally, it possesses a zinc finger DNA-binding domain, a ligand-binding domain, activation domains (AF-1/AF-2), a hinge region, and a fushi tarazu factor 1 (FTZ-F1) box that aids DNA recognition, and its activity is further regulated by phosphorylation, SUMOylation, and acetylation. SF-1 is expressed in the adrenal cortex, gonads, ventromedial hypothalamus, and pituitary gonadotropes from early embryonic stages, where it directs the transcription of genes encoding steroidogenic enzymes such as cytochrome P450 hydroxylases. Functionally, SF-1 is indispensable for adrenal and gonadal development, sex differentiation, pituitary gonadotropin production, and hypothalamic function, with mutations in NR5A1 linked to adrenal insufficiency, gonadal failure, and reproductive disorders.

References
https://pubmed.ncbi.nlm.nih.gov/9183568/ https://pubmed.ncbi.nlm.nih.gov/8187173/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%