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Anti-SERCA2 ATPase Rabbit Antibody [E19A2]

Catalog No.: F4007

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:5000 - 1:10000 IHC1:50 - 1:100 IF1:100 - 1:250 FCM1:100 - 1:1000

Application
WB, IHC, IF , FCM

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
115 kDa 115 kDa, 140 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-SERCA2 ATPase Rabbit Antibody [E19A2] detects endogenous levels of total SERCA2 ATPase protein.

Clone
E19A2

Synonym(s)
ATP2B, ATP2A2, Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, SERCA2, SR Ca(2+)-ATPase 2, Calcium pump 2, Endoplasmic reticulum class 1/2 Ca(2+) ATPase

Background
SERCA2 (Sarco/Endoplasmic Reticulum Ca2+-ATPase 2) is a membrane-bound P-type ATPase predominantly expressed in cardiac and smooth muscle, where it plays a critical role in calcium homeostasis by actively pumping cytosolic Ca2+ into the sarco/endoplasmic reticulum (SR/ER), thus regulating muscle contraction and relaxation. SERCA2 contains ten transmembrane helices creating a channel for Ca2+ transport, along with three cytoplasmic domains (actuator, phosphorylation, and nucleotide-binding domains) responsible for ATP hydrolysis and conformational changes required for active transport. SERCA2 exists mainly as two isoforms, SERCA2a in cardiac muscle and SERCA2b in smooth muscle and non-muscle cells, with differences in their C-terminal sequences influencing their regulation and Ca2+ affinity. SERCA2's ATPase activity couples ATP hydrolysis to Ca2+ translocation, maintaining low cytosolic Ca2+ necessary for muscle relaxation. Its regulation is tightly controlled by accessory proteins such as phospholamban (PLB) and sarcolipin (SLN), which inhibit its Ca2+ affinity and ATPase activity; phosphorylation of PLB and SLN relieves this inhibition during muscle contraction. Post-translational modifications, luminal redox sensing via disulfide bonds, and isoform-specific intramolecular interactions further modulate SERCA2 activity. Dysregulation or decreased expression of SERCA2 is implicated in heart failure and other cardiac pathologies.

Background

SERCA2 (Sarco/Endoplasmic Reticulum Ca2+-ATPase 2) is a membrane-bound P-type ATPase predominantly expressed in cardiac and smooth muscle, where it plays a critical role in calcium homeostasis by actively pumping cytosolic Ca2+ into the sarco/endoplasmic reticulum (SR/ER), thus regulating muscle contraction and relaxation. SERCA2 contains ten transmembrane helices creating a channel for Ca2+ transport, along with three cytoplasmic domains (actuator, phosphorylation, and nucleotide-binding domains) responsible for ATP hydrolysis and conformational changes required for active transport. SERCA2 exists mainly as two isoforms, SERCA2a in cardiac muscle and SERCA2b in smooth muscle and non-muscle cells, with differences in their C-terminal sequences influencing their regulation and Ca2+ affinity. SERCA2's ATPase activity couples ATP hydrolysis to Ca2+ translocation, maintaining low cytosolic Ca2+ necessary for muscle relaxation. Its regulation is tightly controlled by accessory proteins such as phospholamban (PLB) and sarcolipin (SLN), which inhibit its Ca2+ affinity and ATPase activity; phosphorylation of PLB and SLN relieves this inhibition during muscle contraction. Post-translational modifications, luminal redox sensing via disulfide bonds, and isoform-specific intramolecular interactions further modulate SERCA2 activity. Dysregulation or decreased expression of SERCA2 is implicated in heart failure and other cardiac pathologies.

References
https://pubmed.ncbi.nlm.nih.gov/35560336/ https://pubmed.ncbi.nlm.nih.gov/18006443/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%