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Anti-Rho A Mouse Antibody [G4B13]

Catalog No.: F0072

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:100-1:1000 IHC1:50-1:500 IF1:50-1:500 FCM1 µg per 1 x 10⁶ cells

Application
WB, IP, IHC, IF , FCM

Reactivity
Mouse, Rat, Human

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
24 kDa

Datasheet & SDS

Biological Description

Specificity
Anti-Rho A Mouse Antibody [G4B13] detects endogenous levels of total Rho A protein.

Clone
G4B13

Synonym(s)
Transforming protein RhoA, Rho cDNA clone 12 (h12), RHOA, ARH12, ARHA, RHO12

Background
RhoA is a member of the Rho family of small GTPases that functions as a molecular switch to regulate cytoskeletal dynamics, cell shape, motility, and adhesion. RhoA encompasses a conserved GTPase domain that cycles between an inactive GDP-bound and an active GTP-bound state, controlling its interaction with downstream effectors. RhoA’s functions primarily involve activating Rho-associated kinases (ROCK1 and ROCK2), which phosphorylate substrates like myosin light chain (MLC) to promote actomyosin contractility, stress fiber formation, and focal adhesion assembly critical for cell migration and morphogenesis. Regulation of RhoA activity is mediated by guanine nucleotide exchange factors (GEFs) that facilitate GDP-GTP exchange to activate RhoA, GTPase-activating proteins (GAPs) that enhance GTP hydrolysis to inactivate it, and guanine nucleotide dissociation inhibitors (GDIs) that sequester inactive RhoA in the cytosol. Post-translational modifications such as phosphorylation at sites including Ser188 by protein kinase A or ERK and oxidation of cysteine residues modulate RhoA’s activity and localization, influencing processes like tumorigenesis, neurite outgrowth, and vascular function. RhoA also participates in redox-regulated signaling by controlling the production of reactive oxygen species (ROS), which in turn can regulate RhoA activity through feedback mechanisms. RhoA inhibition has been linked to enhanced neurite extension in neuronal cells, while aberrant RhoA activation contributes cancer and cardiovascular diseases.

Background

RhoA is a member of the Rho family of small GTPases that functions as a molecular switch to regulate cytoskeletal dynamics, cell shape, motility, and adhesion. RhoA encompasses a conserved GTPase domain that cycles between an inactive GDP-bound and an active GTP-bound state, controlling its interaction with downstream effectors. RhoA’s functions primarily involve activating Rho-associated kinases (ROCK1 and ROCK2), which phosphorylate substrates like myosin light chain (MLC) to promote actomyosin contractility, stress fiber formation, and focal adhesion assembly critical for cell migration and morphogenesis. Regulation of RhoA activity is mediated by guanine nucleotide exchange factors (GEFs) that facilitate GDP-GTP exchange to activate RhoA, GTPase-activating proteins (GAPs) that enhance GTP hydrolysis to inactivate it, and guanine nucleotide dissociation inhibitors (GDIs) that sequester inactive RhoA in the cytosol. Post-translational modifications such as phosphorylation at sites including Ser188 by protein kinase A or ERK and oxidation of cysteine residues modulate RhoA’s activity and localization, influencing processes like tumorigenesis, neurite outgrowth, and vascular function. RhoA also participates in redox-regulated signaling by controlling the production of reactive oxygen species (ROS), which in turn can regulate RhoA activity through feedback mechanisms. RhoA inhibition has been linked to enhanced neurite extension in neuronal cells, while aberrant RhoA activation contributes cancer and cardiovascular diseases.

References
https://pubmed.ncbi.nlm.nih.gov/33126816/ https://pubmed.ncbi.nlm.nih.gov/28300846/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%