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Anti-Rac1 Mouse Antibody [D22N1]

Catalog No.: F4351

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000

Application
WB, IP, IHC

Reactivity
Mouse, Rat, Human

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
21 kDa

Datasheet & SDS

Biological Description

Specificity
Anti-Rac1 Mouse Antibody [D22N1] recognizes endogenous levels of total Rac1 protein.

Clone
D22N1

Synonym(s)
Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1, RAC1 , TC25

Background
Ras-related C3 botulinum toxin substrate 1 (Rac1) is a small GTPase belonging to the Rac subfamily of the Rho family, widely recognized for its role in cytoskeletal regulation and control of gene expression. Rac1 participates in numerous cellular processes, including cell plasticity, migration, invasion, adhesion, proliferation, apoptosis, production of reactive oxygen species (ROS), and regulation of inflammatory responses—all of which are essential for normal physiology. Disruption of Rac1 signaling can drive disease, as many Rac1-regulated pathways contribute to pathological conditions such as cancer, cardiovascular and kidney diseases, neurodegenerative disorders, aberrant inflammation, and infections. Functionally, Rac1 cycles between an inactive GDP-bound state and an active GTP-bound state. This switch is tightly controlled by guanine nucleotide exchange factors (GEFs), which promote GDP-GTP exchange; GTPase-activating proteins (GAPs), which accelerate GTP hydrolysis; and Rho guanine nucleotide dissociation inhibitors (RhoGDIs), which stabilize the inactive form. In addition to nucleotide cycling, Rac1 activity, stability, and subcellular localization are further regulated through diverse post-translational modifications, ensuring precise spatial and temporal control of Rac1 signaling.

Background

Ras-related C3 botulinum toxin substrate 1 (Rac1) is a small GTPase belonging to the Rac subfamily of the Rho family, widely recognized for its role in cytoskeletal regulation and control of gene expression. Rac1 participates in numerous cellular processes, including cell plasticity, migration, invasion, adhesion, proliferation, apoptosis, production of reactive oxygen species (ROS), and regulation of inflammatory responses—all of which are essential for normal physiology. Disruption of Rac1 signaling can drive disease, as many Rac1-regulated pathways contribute to pathological conditions such as cancer, cardiovascular and kidney diseases, neurodegenerative disorders, aberrant inflammation, and infections. Functionally, Rac1 cycles between an inactive GDP-bound state and an active GTP-bound state. This switch is tightly controlled by guanine nucleotide exchange factors (GEFs), which promote GDP-GTP exchange; GTPase-activating proteins (GAPs), which accelerate GTP hydrolysis; and Rho guanine nucleotide dissociation inhibitors (RhoGDIs), which stabilize the inactive form. In addition to nucleotide cycling, Rac1 activity, stability, and subcellular localization are further regulated through diverse post-translational modifications, ensuring precise spatial and temporal control of Rac1 signaling.

References
https://pubmed.ncbi.nlm.nih.gov/27442895/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%