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Anti-PPP1CB Rabbit Antibody [P21B20]

Catalog No.: F2108

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:50000 IP1:100 IHC1:400 IF1:250 FCM1:20 - 1:50

Application
WB, IP, IHC, IF , FCM

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
37 kDa 38 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Mouse brain; Rat brain; Human fetal brain; Rat kidney tissue; Human cervical carcinoma tissue; Mouse kidney tissue; Human cervical carcinoma; Mouse cardiac muscle; Rat colon; HeLa cell; Jurkat cell
Negative Control

Datasheet & SDS

Biological Description

Specificity
Anti-PPP1CB Rabbit Antibody [P21B20] detects endogenous levels of total PPP1CB protein.

Clone
P21B20

Synonym(s)
Serine/threonine-protein phosphatase PP1-beta catalytic subunit, PP-1B, PPP1CD, PPP1CB

Background
Protein Phosphatase 1 Catalytic Subunit Beta (PPP1CB) is an essential serine/threonine phosphatase isoform within the PP1 family, responsible for catalyzing more than half of all phosphoserine/threonine dephosphorylation reactions in mammalian cells. PPP1CB regulates a wide range of critical cellular processes, including cell cycle progression, protein synthesis, muscle contractility, glycogen metabolism, neuronal signaling, apoptosis, and RNA splicing. It exerts its effects by forming multisubunit holoenzymes in association with numerous regulatory proteins known as PP1-interacting proteins (PIPs), which determine substrate specificity and subcellular localization. This intricate regulation enables PPP1CB to precisely dephosphorylate key substrates such as myosin light chains involved in muscle contraction, glycogen synthase in glucose metabolism, and signaling proteins like AKT and components of the Ras/MAPK pathway, thereby influencing cell proliferation, survival, and cytoskeletal dynamics. PPP1CB plays a crucial role in cytoskeletal rearrangements, essential for migration and angiogenesis in endothelial cells. PPP1CB is ubiquitously expressed at high levels in tissues such as muscle, brain, and heart, where it regulates synaptic plasticity, cardiac contractility, and metabolic homeostasis. Dysregulation of PPP1CB activity is associated with cancer, cardiovascular diseases, and neurodegenerative disorders. Additionally, genetic variants of PPP1CB are linked to developmental disorders, including Noonan-like syndromes.

Background

Protein Phosphatase 1 Catalytic Subunit Beta (PPP1CB) is an essential serine/threonine phosphatase isoform within the PP1 family, responsible for catalyzing more than half of all phosphoserine/threonine dephosphorylation reactions in mammalian cells. PPP1CB regulates a wide range of critical cellular processes, including cell cycle progression, protein synthesis, muscle contractility, glycogen metabolism, neuronal signaling, apoptosis, and RNA splicing. It exerts its effects by forming multisubunit holoenzymes in association with numerous regulatory proteins known as PP1-interacting proteins (PIPs), which determine substrate specificity and subcellular localization. This intricate regulation enables PPP1CB to precisely dephosphorylate key substrates such as myosin light chains involved in muscle contraction, glycogen synthase in glucose metabolism, and signaling proteins like AKT and components of the Ras/MAPK pathway, thereby influencing cell proliferation, survival, and cytoskeletal dynamics. PPP1CB plays a crucial role in cytoskeletal rearrangements, essential for migration and angiogenesis in endothelial cells. PPP1CB is ubiquitously expressed at high levels in tissues such as muscle, brain, and heart, where it regulates synaptic plasticity, cardiac contractility, and metabolic homeostasis. Dysregulation of PPP1CB activity is associated with cancer, cardiovascular diseases, and neurodegenerative disorders. Additionally, genetic variants of PPP1CB are linked to developmental disorders, including Noonan-like syndromes.

References
https://pubmed.ncbi.nlm.nih.gov/22284538/ https://pubmed.ncbi.nlm.nih.gov/34125004/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%