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Anti-PON2 Rabbit Antibody [K8P11]

Catalog No.: F3855

Application: Reactivity: Human

Usage Information

Dilution
WB1:10000 - 1:50000 IP1:50 - 1:70 IHC1:100

Application
WB, IP, IHC

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
39 kDa 39,41 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-PON2 Rabbit Antibody [K8P11] detects endogenous levels of total PON2 protein.

Clone
K8P11

Synonym(s)
Serum paraoxonase/arylesterase 2, PON 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2, A-esterase 2, PON2

Background
Paraoxonase 2 (PON2) is a critical intracellular enzyme predominantly localized to the mitochondria and endoplasmic reticulum, where it plays a vital role in mitigating oxidative stress and preventing apoptosis. PON2 is a type II transmembrane protein, featuring an N-terminal membrane anchor and a catalytic domain with calcium-dependent lactonase activity. PON2 reduces cellular reactive oxygen species (ROS) by interacting with coenzyme Q and modulating mitochondrial respiration, thus preventing lipid peroxidation and preserving mitochondrial integrity. This antioxidant function is especially important in tissues with high oxidative activity, such as the vascular endothelium, brain, heart, and liver. PON2 also interferes with bacterial quorum-sensing molecules, thereby reducing infection-induced oxidative stress. Its expression is tightly regulated by oxidative stress, inflammatory signals, and various transcription factors, including Nrf2, PPAR-γ, AP-1, and NF-κB, which modulate its transcription in response to changes in cellular redox state and inflammation. Post-translational modifications such as glycosylation, phosphorylation, and ubiquitination further regulate PON2’s stability and enzymatic activity. PON2 expression shows sexual dimorphism, with females generally exhibiting higher levels, potentially influencing differential disease susceptibility. Genetic polymorphisms in PON2 can alter its enzymatic activity, and these variations results in the development of cardiovascular diseases, neurodegenerative disorders, and cancer.

Background

Paraoxonase 2 (PON2) is a critical intracellular enzyme predominantly localized to the mitochondria and endoplasmic reticulum, where it plays a vital role in mitigating oxidative stress and preventing apoptosis. PON2 is a type II transmembrane protein, featuring an N-terminal membrane anchor and a catalytic domain with calcium-dependent lactonase activity. PON2 reduces cellular reactive oxygen species (ROS) by interacting with coenzyme Q and modulating mitochondrial respiration, thus preventing lipid peroxidation and preserving mitochondrial integrity. This antioxidant function is especially important in tissues with high oxidative activity, such as the vascular endothelium, brain, heart, and liver. PON2 also interferes with bacterial quorum-sensing molecules, thereby reducing infection-induced oxidative stress. Its expression is tightly regulated by oxidative stress, inflammatory signals, and various transcription factors, including Nrf2, PPAR-γ, AP-1, and NF-κB, which modulate its transcription in response to changes in cellular redox state and inflammation. Post-translational modifications such as glycosylation, phosphorylation, and ubiquitination further regulate PON2’s stability and enzymatic activity. PON2 expression shows sexual dimorphism, with females generally exhibiting higher levels, potentially influencing differential disease susceptibility. Genetic polymorphisms in PON2 can alter its enzymatic activity, and these variations results in the development of cardiovascular diseases, neurodegenerative disorders, and cancer.

References
https://pubmed.ncbi.nlm.nih.gov/35092416/ https://pubmed.ncbi.nlm.nih.gov/33562328/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%