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Anti-PMCA ATPase Mouse Antibody [C12G10]

Catalog No.: F2711

Application: Reactivity: Human, Mouse, Rat, Amphibian, Dog, Chicken, Cat, Non-human primate, Sheep, Rabbit

Usage Information

Dilution
WB1:1000 - 1:5000 IHC1:500

Application
WB, IP, IHC, IF, FCM

Reactivity
Human, Mouse, Rat, Amphibian, Dog, Chicken, Cat, Non-human primate, Sheep, Rabbit

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
~140 kDa

Datasheet & SDS

Biological Description

Specificity
Anti-PMCA ATPase Mouse Antibody [C12G10] detects endogenous levels of total PMCA ATPase protein.

Clone
C12G10

Synonym(s)
ATP-dependent plasma membrane calcium transporter; ATPase isoform 2 Na+K+ transporting beta polypeptide 2; ATPase isoform 2, Na+K+ transporting, beta polypeptide 2; ATPase, Ca++ transporting, plasma membrane 1; ATPase, Ca++ transporting, plasma membrane 2; ATPase, Ca++ transporting, plasma membrane 3

Background
PMCA ATPase are P-type ATP-driven ion pumps that maintain calcium homeostasis by actively extruding Ca²⁺ from the cytosol to the extracellular space, thereby sustaining low resting intracellular calcium levels essential for cellular signaling. Structurally, PMCAs are integral membrane proteins composed of 10 transmembrane domains, with large intracellular loops containing the catalytic ATP-binding site, and cytoplasmic N- and C-terminal domains that regulate pump activity and mediate protein–protein interactions. They are encoded by four genes (PMCA1–4): PMCA1 and PMCA4 are ubiquitously expressed across tissues, whereas PMCA2 and PMCA3 are enriched in excitable cells such as neurons, sensory epithelia, and muscle. Beyond their canonical role as calcium transporters, PMCAs also act as modulators of signal transduction by creating localized low-calcium microdomains and scaffolding signaling complexes through interactions with PDZ domain-containing and cytoskeletal proteins. This dual function—calcium extrusion and signal compartmentalization—makes PMCAs critical for diverse physiological processes, including synaptic transmission, cardiovascular regulation, and calcium-dependent enzyme activity.

Background

PMCA ATPase are P-type ATP-driven ion pumps that maintain calcium homeostasis by actively extruding Ca²⁺ from the cytosol to the extracellular space, thereby sustaining low resting intracellular calcium levels essential for cellular signaling. Structurally, PMCAs are integral membrane proteins composed of 10 transmembrane domains, with large intracellular loops containing the catalytic ATP-binding site, and cytoplasmic N- and C-terminal domains that regulate pump activity and mediate protein–protein interactions. They are encoded by four genes (PMCA1–4): PMCA1 and PMCA4 are ubiquitously expressed across tissues, whereas PMCA2 and PMCA3 are enriched in excitable cells such as neurons, sensory epithelia, and muscle. Beyond their canonical role as calcium transporters, PMCAs also act as modulators of signal transduction by creating localized low-calcium microdomains and scaffolding signaling complexes through interactions with PDZ domain-containing and cytoskeletal proteins. This dual function—calcium extrusion and signal compartmentalization—makes PMCAs critical for diverse physiological processes, including synaptic transmission, cardiovascular regulation, and calcium-dependent enzyme activity.

References
https://pubmed.ncbi.nlm.nih.gov/21537369/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%