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Anti-Phospho-PKC (Ser660) Rabbit Antibody [J16E1]

Catalog No.: F2745

Application: Reactivity: Human

Usage Information

Dilution
WB1:50000 - 1:200000 IHC1:100 - 1:250

Application
WB, IHC

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
77 kDa 77 kDa, 100 kDa-150 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Anti-Phospho-PKC (Ser660) Rabbit Antibody [J16E1] detects endogenous levels of PKC only when phosphorylated at a carboxy-terminal residue homologous to serine 660.

Clone
J16E1

Synonym(s)
PKCA, PRKACA, PRKCA, Protein kinase C alpha type, PKC-A, PKC-alpha

Background
Phospho-PKC (Ser660) refers to the phosphorylated form of protein kinase C βII (PKCβII) at serine residue 660, a key site within its C-terminal hydrophobic motif that is critical for kinase maturation and activity. Structurally, Ser660 lies adjacent to Thr641 in the C-terminal tail and, together with Thr500 in the activation loop, forms one of the three conserved phosphorylation sites essential for PKC regulation. Phosphorylation at Ser660 occurs via autophosphorylation after prior modification at Thr641 and stabilizes the enzyme in a catalytically competent conformation while also influencing its subcellular localization, including release into the cytosol for interaction with lipid second messengers such as diacylglycerol. PKC isoforms, including PKCβII, are widely expressed across tissues and regulate diverse processes such as cell growth, migration, metabolism, and immune signaling. Importantly, phosphorylation at Ser660 fine-tunes PKC activation, ensuring proper responsiveness to signals while preventing premature or uncontrolled activity. Dysregulation of this phosphorylation event has been linked to pathological states including cancer, diabetes, and cardiovascular diseases, underscoring its essential role in maintaining balanced PKC signaling.

Background

Phospho-PKC (Ser660) refers to the phosphorylated form of protein kinase C βII (PKCβII) at serine residue 660, a key site within its C-terminal hydrophobic motif that is critical for kinase maturation and activity. Structurally, Ser660 lies adjacent to Thr641 in the C-terminal tail and, together with Thr500 in the activation loop, forms one of the three conserved phosphorylation sites essential for PKC regulation. Phosphorylation at Ser660 occurs via autophosphorylation after prior modification at Thr641 and stabilizes the enzyme in a catalytically competent conformation while also influencing its subcellular localization, including release into the cytosol for interaction with lipid second messengers such as diacylglycerol. PKC isoforms, including PKCβII, are widely expressed across tissues and regulate diverse processes such as cell growth, migration, metabolism, and immune signaling. Importantly, phosphorylation at Ser660 fine-tunes PKC activation, ensuring proper responsiveness to signals while preventing premature or uncontrolled activity. Dysregulation of this phosphorylation event has been linked to pathological states including cancer, diabetes, and cardiovascular diseases, underscoring its essential role in maintaining balanced PKC signaling.

References
https://pubmed.ncbi.nlm.nih.gov/8749392/ https://pubmed.ncbi.nlm.nih.gov/21215369/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%